| Harman Brothers Ice, LLC | Wholesale Trade | $630,300 | $679,747 | 60 | $15.4M |  AI Deep Dive |
| Appalachia Service Project INC | Health Care and Social Assistance | $623,505 | $627,107 | 59 | N/A | AI Deep Dive |
| Johnson City Urological Clinic PC | Health Care and Social Assistance | $621,258 | $629,300 | 54 | $16.9M | AI Deep Dive |
| Pain Medicine Associates PC | Health Care and Social Assistance | $616,077 | $623,265 | 56 | $17.6M | AI Deep Dive |
| Northwest Tennessee Automotive Group, LLC | Retail Trade | $614,300 | $618,323 | 46 | $11.6M | AI Deep Dive |
| Appalachia Service Project INC | Other Services (except Public Administration) | $613,090 | $617,399 | 59 | N/A | AI Deep Dive |
| East Tennessee Rent-Alls Incorporated | Real Estate and Rental and Leasing | $608,458 | $613,725 | 57 | $25.8M | AI Deep Dive |
| C Gatton, Inc. | Retail Trade | $593,300 | $596,747 | 48 | $12.1M | AI Deep Dive |
| Fiber Innovation Technology, Inc. | Manufacturing | $589,532 | $592,365 | 52 | $18.4M | AI Deep Dive |
| Clw Delivery, Inc. | Transportation and Warehousing | $574,411 | $577,502 | 67 | $15.6M | AI Deep Dive |
| Minifibers, Inc. | Manufacturing | $573,973 | $579,492 | 49 | $17.3M | AI Deep Dive |
| Agape Nursing & Rehabilitation Center, LLC | Health Care and Social Assistance | $568,362 | $573,303 | 70 | $11.9M | AI Deep Dive |
| Providence Academy | Educational Services | $566,200 | $539,998 | 36 | <$5M | AI Deep Dive |
| The Waters of Johnson City, LLC | Health Care and Social Assistance | $563,533 | $573,410 | 84 | $14.3M | AI Deep Dive |
| Jd Squared Inc. | Manufacturing | $552,332 | $556,965 | 50 | $13.1M | AI Deep Dive |
| Karing Hearts Cardiology, Pllc | Health Care and Social Assistance | $543,676 | $549,052 | 46 | $14.4M | AI Deep Dive |
| Enhanced Support Services, Inc. | Other Services (except Public Administration) | $541,750 | $546,641 | 140 | $26.7M | AI Deep Dive |
| Ear, Nose & Throat Associates PC | Health Care and Social Assistance | $532,900 | $539,310 | 49 | $15.4M | AI Deep Dive |
| Watauga Pathology Associates P C | Health Care and Social Assistance | $509,115 | $512,778 | 28 | $8.8M | AI Deep Dive |
| Chaparral Buick-Gmc Truck, Inc. | Retail Trade | $477,995 | $482,496 | 47 | $11.8M | AI Deep Dive |
| Summit Automotive, Inc. | Retail Trade | $477,400 | $481,975 | 46 | $11.6M | AI Deep Dive |
| Jd Squared, Inc. | Manufacturing | $463,957 | $468,081 | 49 | $12.0M | AI Deep Dive |
| Jdd Enterprises INC | Accommodation and Food Services | $462,452 | $465,958 | 37 | $6.6M | AI Deep Dive |
| Recdirect INC | Wholesale Trade | $459,890 | $463,441 | 34 | $10.4M | AI Deep Dive |
| Whitson Company, INC | Wholesale Trade | $459,870 | $464,571 | 21 | $6.4M | AI Deep Dive |