| Copper Celler Corporation | Accommodation and Food Services | $7,670,500 | $7,769,151 | 500 | $88.8M |  Research |
| Orthotennessee P.c. | Health Care and Social Assistance | $7,531,800 | $7,612,896 | 500 | $156.9M | Research |
| Connor Concepts, Inc. | Accommodation and Food Services | $7,046,400 | $7,139,065 | 500 | $88.8M | Research |
| Inmet Mining, LLC | Mining, Quarrying, and Oil and Gas Extraction | $6,833,400 | $5,604,668 | 373 | $22.6M | Research |
| Mortgage Investors Group INC | Finance and Insurance | $6,160,385 | $6,026,806 | 394 | $202.4M | Research |
| Aubrey's INC | Accommodation and Food Services | $5,135,200 | $5,195,539 | 500 | $88.8M | Research |
| Diocese of Knoxville | Other Services (except Public Administration) | $5,059,600 | $4,178,024 | 499 | N/A | Research |
| Cherokee Distributing Company, Inc. | Wholesale Trade | $4,921,000 | $4,976,816 | 403 | $103.7M | Research |
| Holston Gases, Inc. | Wholesale Trade | $4,663,870 | $4,717,375 | 424 | $129.9M | Research |
| Superior Steel, Inc. | Construction | $3,993,300 | $4,043,079 | 285 | $88.3M | Research |
| Presbyterian Homes of Tennessee, INC | Health Care and Social Assistance | $3,934,505 | $4,008,058 | 500 | N/A | Research |
| Gem Technologies, INC | Administrative and Support and Waste Management | $3,900,000 | $3,944,417 | 279 | $52.8M | Research |
| Alliance for Multispecialty Research, LLC | Professional, Scientific, and Technical Services | $3,615,400 | $3,658,488 | 294 | $61.1M | Research |
| Vanquish Worldwide LLC | Administrative and Support and Waste Management | $3,544,068 | $3,585,022 | 376 | $71.1M | Research |
| Pya, P.c. | Professional, Scientific, and Technical Services | $3,498,855 | $3,539,092 | 231 | $73.5M | Research |
| Allergy Associates, P.a. | Health Care and Social Assistance | $3,492,313 | $3,531,733 | 340 | $106.7M | Research |
| Newstat, Pllc | Health Care and Social Assistance | $3,468,831 | $3,509,590 | 171 | $53.7M | Research |
| Edfinancial Services, LLC | Finance and Insurance | $3,392,400 | $1,505,501 | 380 | $195.2M | Research |
| Tennessee Orthopaedic Clinics, PC | Health Care and Social Assistance | $3,356,700 | $3,395,601 | 323 | $101.4M | Research |
| Security Walls LLC | Administrative and Support and Waste Management | $3,133,084 | $3,173,514 | 250 | $36.6M | Research |
| Arc Automotive, Inc. | Manufacturing | $2,935,062 | $2,967,185 | 277 | $72.5M | Research |
| House-Hasson Hardware Company | Wholesale Trade | $2,842,599 | $2,881,053 | 255 | $78.1M | Research |
| Shoney's of Knoxville, Inc. | Accommodation and Food Services | $2,791,900 | $2,841,313 | 500 | $88.8M | Research |
| Tennessee Cancer Specialists, Pllc | Health Care and Social Assistance | $2,771,255 | $2,811,053 | 250 | $78.5M | Research |
| Magnum Venus Products, Inc. | Manufacturing | $2,714,200 | $2,744,316 | 259 | $256.9M | Research |