| Chota Community Health Services | Other Services (except Public Administration) | $1,018,847 | $1,024,479 | 90 | N/A |  Research |
| Madisonville Health and Rehabilitation LLC | Health Care and Social Assistance | $512,810 | $0 | 68 | $11.6M | Research |
| Madisonville Healthcare, LLC | Health Care and Social Assistance | $512,617 | $517,629 | 72 | N/A | Research |
| Sloan of Vonore | Retail Trade | $398,296 | $402,478 | 77 | $25.8M | Research |
| Warrenjacksoncpas, Pllc | Professional, Scientific, and Technical Services | $273,350 | $275,241 | 15 | <$5M | Research |
| I.c.e. Packaging Company, LLC | Administrative and Support and Waste Management | $225,000 | $227,114 | 25 | <$5M | Research |
| Biereley-Hale Funeral Home, Incorporated | Retail Trade | $214,360 | $216,909 | 14 | <$5M | Research |
| Traffic Pro, LLC | Professional, Scientific, and Technical Services | $212,010 | $214,419 | 20 | <$5M | Research |
| Monroe Company Inc. | Accommodation and Food Services | $210,921 | $212,731 | 47 | $8.3M | Research |
| Hyde Enterprise, INC | Administrative and Support and Waste Management | $209,183 | $211,890 | 48 | $7.6M | Research |
| USA Strength and Performance LLC | Manufacturing | $205,400 | $207,705 | 37 | $9.0M | Research |
| Stricklan Tree Service, INC | Agriculture, Forestry, Fishing and Hunting | $165,683 | $166,958 | 24 | <$5M | Research |
| Access Medical Care of Monroe County P.c. | Health Care and Social Assistance | $163,314 | $164,852 | 27 | $8.5M | Research |
| Benton's Smoky Mountain Country Hams, Inc. | Retail Trade | $163,067 | $164,027 | 20 | <$5M | Research |
| Madisonville Marine INC | Retail Trade | $149,500 | $150,770 | 14 | <$5M | Research |
| Monroe Company | Accommodation and Food Services | $148,470 | $149,361 | 28 | <$5M | Research |
| Access Medical Care of Monroe County, P.c. | Health Care and Social Assistance | $147,040 | $147,943 | 24 | $7.5M | Research |
| Cooper Services, LLC | Manufacturing | $141,420 | $142,983 | 27 | $6.6M | Research |
| Jbac Fitness, INC | Arts, Entertainment, and Recreation | $123,100 | $123,906 | 15 | <$5M | Research |
| Jbac Fitness | Arts, Entertainment, and Recreation | $122,954 | $123,415 | 15 | <$5M | Research |
| J & a Ogle Enterprises, INC | Accommodation and Food Services | $111,717 | $112,427 | 39 | $6.9M | Research |
| Skyway Title Services | Professional, Scientific, and Technical Services | $105,600 | $106,193 | 8 | <$5M | Research |
| T&d Restaurant Management LLC | Accommodation and Food Services | $105,526 | $106,461 | 35 | $6.2M | Research |
| Family Dental Associates, P.c. | Health Care and Social Assistance | $102,408 | $103,557 | 11 | <$5M | Research |
| Donna's Cafe LLC | Accommodation and Food Services | $94,530 | $95,494 | 20 | <$5M | Research |