| Paramont Mfg LLC | Manufacturing | $3,984,800 | $4,029,740 | 316 | $82.7M |  Research |
| Penn Stuart & Eskridge | Professional, Scientific, and Technical Services | $1,738,800 | $1,750,471 | 108 | $26.2M | Research |
| Universal Companies, Inc. | Management of Companies and Enterprises | $1,524,257 | $1,542,590 | 132 | $21.7M | Research |
| Universal Companies Inc. | Management of Companies and Enterprises | $1,524,255 | $1,536,195 | 108 | $17.8M | Research |
| General Engineering Co of Virginia | Professional, Scientific, and Technical Services | $1,457,100 | $1,471,023 | 120 | $14.8M | Research |
| General Engineering Company of Virginia | Manufacturing | $1,440,345 | $1,449,307 | 193 | $47.2M | Research |
| Acp LLC | Manufacturing | $1,118,705 | $1,122,322 | 118 | $30.9M | Research |
| Acp, LLC | Manufacturing | $1,118,705 | $1,126,381 | 118 | $30.9M | Research |
| Mxi Express Inc. | Administrative and Support and Waste Management | $875,340 | $881,638 | 73 | $13.8M | Research |
| Patriot Mining, LLC | Mining, Quarrying, and Oil and Gas Extraction | $866,800 | $871,832 | 57 | <$5M | Research |
| Mxi Express INC | Transportation and Warehousing | $832,937 | $840,966 | 70 | $10.6M | Research |
| The Barter Foundation, Incorporated | Arts, Entertainment, and Recreation | $795,100 | $587,368 | 104 | $17.1M | Research |
| Blue Mountain Therapy | Health Care and Social Assistance | $762,518 | $772,134 | 84 | $22.7M | Research |
| Dutt & Wagner of Virginia INC | Wholesale Trade | $748,500 | $756,671 | 69 | $17.8M | Research |
| Virginia Highlands Anesthesia, PC | Health Care and Social Assistance | $629,500 | $635,533 | 29 | $9.1M | Research |
| Wolf Hills Fabricators LLC | Manufacturing | $604,840 | $609,796 | 50 | $12.2M | Research |
| Wolf Hills Fabricators, LLC | Manufacturing | $604,800 | $608,585 | 49 | $12.8M | Research |
| Roger's Trucking Inc. | Transportation and Warehousing | $503,800 | $506,809 | 63 | $9.5M | Research |
| Pioneer Chevrolet Inc. | Retail Trade | $493,500 | $499,833 | 53 | $13.4M | Research |
| Pioneer Chevrolet Inc. | Retail Trade | $493,500 | $497,818 | 51 | $12.9M | Research |
| Highlands Automotive, Inc. | Retail Trade | $487,919 | $492,188 | 48 | $12.1M | Research |
| Buckhead-Abingdon LLC | Accommodation and Food Services | $486,073 | $489,543 | 66 | $20.6M | Research |
| Highlands Fellowship | Other Services (except Public Administration) | $442,620 | $445,030 | 71 | N/A | Research |
| Green Valley Poultry Farm INC | Agriculture, Forestry, Fishing and Hunting | $404,500 | $408,916 | 54 | $8.8M | Research |
| Matney Construction Company INC | Mining, Quarrying, and Oil and Gas Extraction | $381,200 | $384,398 | 12 | <$5M | Research |