| Jay M Bass DDS PC | Health Care and Social Assistance | $100,000 | $100,690 | 14 | <$5M | AI Deep Dive |
| Tnt Flinchum Property Managment Corporation | Real Estate and Rental and Leasing | $99,300 | $100,067 | 9 | <$5M | AI Deep Dive |
| Jb Brewing INC | Accommodation and Food Services | $97,060 | $97,823 | 11 | <$5M | AI Deep Dive |
| Gillies INC | Accommodation and Food Services | $96,600 | $97,145 | 14 | <$5M | AI Deep Dive |
| Blacksburg Wine Lab INC | Accommodation and Food Services | $96,516 | $97,225 | 25 | <$5M | AI Deep Dive |
| Emisshield Inc. | Professional, Scientific, and Technical Services | $96,090 | $96,562 | 5 | <$5M | AI Deep Dive |
| Matthew T Ankrum DDS Ms PC | Health Care and Social Assistance | $95,524 | $96,233 | 8 | <$5M | AI Deep Dive |
| 107 Success LLC | Administrative and Support and Waste Management | $95,100 | $96,124 | 14 | <$5M | AI Deep Dive |
| Peacock Holland Construction LLC | Construction | $94,100 | $94,886 | 8 | <$5M | AI Deep Dive |
| Richard L. Anthony, DDS | Health Care and Social Assistance | $93,600 | $94,604 | 9 | <$5M | AI Deep Dive |
| 1200 West LLC | Health Care and Social Assistance | $93,590 | $94,284 | 10 | <$5M | AI Deep Dive |
| A and K Cares, INC | Health Care and Social Assistance | $93,500 | $94,391 | 24 | <$5M | AI Deep Dive |
| Johnston Enterprises, Inc. | Wholesale Trade | $93,197 | $0 | 59 | $18.1M | AI Deep Dive |
| Dayspring Christian Schools Inc. | Educational Services | $92,200 | $92,743 | 24 | N/A | AI Deep Dive |
| Fitzwater.inc | Other Services (except Public Administration) | $91,264 | $91,922 | 10 | <$5M | AI Deep Dive |
| Vantage Human Resources Services Inc. | Professional, Scientific, and Technical Services | $91,045 | $91,599 | 12 | <$5M | AI Deep Dive |
| Cascade Lodging LLC | Accommodation and Food Services | $91,000 | $91,681 | 9 | <$5M | AI Deep Dive |
| Techulon INC | Professional, Scientific, and Technical Services | $90,630 | $91,327 | 6 | <$5M | AI Deep Dive |
| Sheeflee LLC | Construction | $90,113 | $90,448 | 4 | <$5M | AI Deep Dive |
| East Coasters Bike Shop Inc. | Manufacturing | $89,900 | $90,664 | 13 | <$5M | AI Deep Dive |
| Blacksburg Hospitality Group LLC | Accommodation and Food Services | $89,700 | $90,139 | 9 | <$5M | AI Deep Dive |
| New Oasis Market Inc. | Retail Trade | $89,400 | $90,056 | 12 | <$5M | AI Deep Dive |
| Michael J. Ankrum DDS PC | Health Care and Social Assistance | $89,200 | $90,251 | 7 | <$5M | AI Deep Dive |
| Blacksburg Baptist Church | Other Services (except Public Administration) | $88,607 | $89,451 | 34 | N/A | AI Deep Dive |
| Colliver Sports and Spine INC | Health Care and Social Assistance | $88,125 | $88,717 | 8 | <$5M | AI Deep Dive |