| Petersburg Motor Company, INC | Retail Trade | $4,900,700 | $4,958,283 | 367 | $92.5M |  Research |
| Red Light Management LLC | Arts, Entertainment, and Recreation | $4,889,800 | $4,947,527 | 287 | $47.1M | Research |
| Faulconer Construction Company INC | Construction | $4,288,791 | $4,341,432 | 372 | $115.3M | Research |
| Apex Clean Energy INC | Professional, Scientific, and Technical Services | $4,101,500 | $4,154,478 | 216 | $22.4M | Research |
| St Annes Belfield INC | Educational Services | $2,882,500 | $2,915,696 | 337 | N/A | Research |
| Thomas Jefferson Foundation INC | Arts, Entertainment, and Recreation | $2,837,400 | $2,869,601 | 353 | $53.7M | Research |
| University of Virginia Host Properties INC | Accommodation and Food Services | $2,700,000 | $2,732,025 | 250 | $78.2M | Research |
| Silverchair Science & Communications, LLC | Information | $2,592,800 | $2,622,257 | 145 | $24.4M | Research |
| Commonwealth Computer Research INC | Professional, Scientific, and Technical Services | $2,562,634 | $305,558 | 135 | $27.9M | Research |
| Design Electric INC | Construction | $2,476,000 | $2,518,780 | 228 | $75.0M | Research |
| Tiger Fuel Company | Retail Trade | $2,413,900 | $2,440,721 | 270 | $64.5M | Research |
| Teachstone, Inc. | Educational Services | $2,347,800 | $2,375,191 | 128 | $17.1M | Research |
| Haslup Enterprises Ltd. | Health Care and Social Assistance | $2,120,700 | $2,145,854 | 290 | $49.7M | Research |
| Accounting Research & Analytics, LLC | Unknown | $2,073,042 | $2,097,026 | 500 | N/A | Research |
| Natural Retreats US LLC | Real Estate and Rental and Leasing | $2,063,600 | $2,088,421 | 150 | $22.5M | Research |
| Elder Research INC | Professional, Scientific, and Technical Services | $1,975,000 | $1,658,800 | 90 | $9.3M | Research |
| S. L. Williamson Co., Inc. | Manufacturing | $1,971,700 | $1,990,541 | 159 | $157.7M | Research |
| Coconstruct, LLC | Professional, Scientific, and Technical Services | $1,961,500 | $0 | 126 | $26.1M | Research |
| S. L. Williamson Co. Inc. | Manufacturing | $1,958,750 | $1,971,210 | 159 | $157.7M | Research |
| University of Virginia Darden School Foundation | Other Services (except Public Administration) | $1,902,600 | $1,922,630 | 84 | N/A | Research |
| Anchor Healthcare, P.l.c. | Health Care and Social Assistance | $1,815,800 | $1,833,908 | 114 | $35.8M | Research |
| University of Virginia Darden School Foundation | Finance and Insurance | $1,802,623 | $1,815,491 | 63 | N/A | Research |
| Hospice of the Piedmont, Inc. | Health Care and Social Assistance | $1,764,700 | $1,782,641 | 187 | N/A | Research |
| Atlantic Coast Athletic Clubs of Virginia, Inc. | Arts, Entertainment, and Recreation | $1,697,600 | $1,714,623 | 179 | $25.1M | Research |
| Dlb Enterprises LLC | Construction | $1,594,201 | $1,606,999 | 107 | $121.9M | Research |