| Musictoday Ii, LLC | Retail Trade | $1,502,100 | $1,513,700 | 143 | $35.7M |  Research |
| Otpr Management LLC | Other Services (except Public Administration) | $1,158,665 | $1,164,812 | 194 | $37.1M | Research |
| R a Yancey Lumber Corp | Manufacturing | $830,800 | $837,151 | 75 | $74.4M | Research |
| The Willis Organization LLC | Administrative and Support and Waste Management | $613,436 | $617,889 | 60 | $11.4M | Research |
| The Willis Organization, LLC | Administrative and Support and Waste Management | $540,900 | $544,960 | 49 | $9.3M | Research |
| Otpr Management LLC | Other Services (except Public Administration) | $462,500 | $467,202 | 49 | $9.4M | Research |
| Starr Hill Brewery, LLC | Manufacturing | $430,600 | $434,751 | 49 | $17.3M | Research |
| Counterflow Ai INC | Professional, Scientific, and Technical Services | $349,000 | $352,829 | 14 | <$5M | Research |
| Innisfree Incorporated | Health Care and Social Assistance | $267,068 | $268,452 | 38 | N/A | Research |
| King Family Vineyards LLC | Retail Trade | $256,980 | $258,860 | 25 | <$5M | Research |
| Old Trail Golf Club, LLC | Arts, Entertainment, and Recreation | $252,200 | $254,470 | 26 | <$5M | Research |
| Clear Path Communications INC | Construction | $250,705 | $253,191 | 17 | $5.6M | Research |
| Clear Path Communications, INC | Construction | $247,187 | $248,835 | 19 | $6.3M | Research |
| Fardowners Restaurant Inc. | Accommodation and Food Services | $245,669 | $249,088 | 19 | <$5M | Research |
| Smoked Kitchen and Tap LLC | Accommodation and Food Services | $212,456 | $214,982 | 33 | $5.9M | Research |
| Crozet Pizza LLC | Accommodation and Food Services | $199,898 | $202,252 | 18 | <$5M | Research |
| Perrone Robotics, Incorporated | Professional, Scientific, and Technical Services | $191,365 | $193,151 | 13 | <$5M | Research |
| Animal Wellness Center | Professional, Scientific, and Technical Services | $189,105 | $190,954 | 22 | <$5M | Research |
| Fardowners Restaurant Inc. | Accommodation and Food Services | $171,800 | $174,024 | 37 | $6.6M | Research |
| Lucindas LLC | Other Services (except Public Administration) | $162,000 | $165,650 | 12 | <$5M | Research |
| Staengl Engineering LLC | Professional, Scientific, and Technical Services | $146,900 | $148,349 | 11 | <$5M | Research |
| Sentry Electric Company Inc. | Construction | $142,500 | $143,464 | 14 | <$5M | Research |
| Virginia Asphalt Services, Inc. | Manufacturing | $141,632 | $142,619 | 14 | $13.9M | Research |
| Sentry Electric Company Inc. | Construction | $140,300 | $141,949 | 15 | <$5M | Research |
| Field School of Charlottesville | Educational Services | $135,295 | $136,270 | 19 | N/A | Research |