| Tidewater Physicians Multispecialty Group, P.c. | Health Care and Social Assistance | $10,000,000 | $10,126,667 | 500 | $156.9M |  Research |
| Pbmares, LLP | Professional, Scientific, and Technical Services | $5,570,800 | $5,637,031 | 307 | $97.7M | Research |
| W. M. Jordan Company, Inc. | Construction | $5,470,100 | $5,536,653 | 309 | $352.1M | Research |
| Warwick Plumbing and Heating Corporation | Construction | $5,105,200 | $1,861,819 | 409 | $129.4M | Research |
| Young Men's Christian Association of the Virginia Peninsulas | Other Services (except Public Administration) | $2,455,980 | $2,471,193 | 125 | N/A | Research |
| Hope in Home Care LLC | Health Care and Social Assistance | $2,227,000 | $2,246,464 | 437 | $74.9M | Research |
| Sree Siva Sai IV LLC | Accommodation and Food Services | $2,201,100 | $22,869 | 8 | <$5M | Research |
| R & K Enterprise Solutions, INC | Professional, Scientific, and Technical Services | $2,174,300 | $2,198,902 | 161 | $33.3M | Research |
| Hampton Roads Community Action Program, INC | Health Care and Social Assistance | $2,000,000 | $2,019,500 | 319 | N/A | Research |
| R & K Enterprise Solutions INC | Professional, Scientific, and Technical Services | $2,000,000 | $2,018,849 | 91 | $9.4M | Research |
| Drucker & Falk, LLC | Real Estate and Rental and Leasing | $1,990,500 | $2,013,944 | 116 | $21.2M | Research |
| Spectrum Comm INC | Professional, Scientific, and Technical Services | $1,989,300 | $2,008,806 | 131 | $27.1M | Research |
| C & F Enterprises, Inc. | Wholesale Trade | $1,947,600 | $1,972,973 | 255 | $78.1M | Research |
| Newport New Baptist Retirement Community | Health Care and Social Assistance | $1,828,682 | $1,849,966 | 310 | N/A | Research |
| Enterprise Leasing LLC | Information | $1,812,989 | $0 | 241 | $40.5M | Research |
| Davis Boat Works | Manufacturing | $1,812,200 | $1,830,272 | 124 | $32.5M | Research |
| Power Mechanical, Inc. | Manufacturing | $1,663,000 | $1,671,989 | 116 | $30.4M | Research |
| Peninsula Motor Cars Inc. | Retail Trade | $1,584,800 | $1,597,743 | 142 | $35.8M | Research |
| Orthopaedic Surgery & Sports Medicine Specialists of Hampton | Health Care and Social Assistance | $1,572,900 | $1,594,178 | 144 | $45.2M | Research |
| Carr Electrical Technology INC | Construction | $1,561,883 | $1,574,899 | 98 | $111.7M | Research |
| Carr Electrical Technology, Inc. | Construction | $1,561,883 | $1,578,065 | 98 | $32.2M | Research |
| Orthopaedic Surgery & Sports Medicine Specialists of Hampton | Health Care and Social Assistance | $1,556,000 | $1,575,061 | 136 | $42.7M | Research |
| Hampton Roads Academy | Other Services (except Public Administration) | $1,545,900 | $1,555,476 | 254 | N/A | Research |
| Suttle Motor Corporation | Retail Trade | $1,540,000 | $1,549,411 | 137 | $34.5M | Research |
| Hampton Roads Orthopaedics and Sports Medicine LLC | Health Care and Social Assistance | $1,466,600 | $1,479,066 | 122 | $38.3M | Research |