| Colonna's Ship Yard, Incorporated | Manufacturing | $10,000,000 | $10,128,333 | 500 | $130.9M |  Research |
| Evms Academic Physicians and Surgeons Health Services Founda | Health Care and Social Assistance | $7,976,600 | $0 | 500 | N/A | Research |
| Tp Host LLC | Transportation and Warehousing | $7,970,780 | $8,000,228 | 496 | $38.6M | Research |
| Lombart Brothers INC | Wholesale Trade | $5,505,597 | $5,586,748 | 308 | $94.4M | Research |
| Virginia Oncology Associates | Health Care and Social Assistance | $5,501,347 | $5,584,696 | 353 | $110.8M | Research |
| Children's Specialty Group | Health Care and Social Assistance | $4,223,700 | $4,278,021 | 249 | $78.2M | Research |
| East Coast Repair & Fabrication LLC | Manufacturing | $4,170,600 | $3,274,117 | 350 | $91.6M | Research |
| Kaufman & Canoles, P.c. | Professional, Scientific, and Technical Services | $4,156,400 | $4,206,854 | 230 | $55.9M | Research |
| Lyon Shipyard, Inc. | Manufacturing | $3,625,199 | $3,665,076 | 248 | $64.9M | Research |
| Xylem, Inc. | Administrative and Support and Waste Management | $3,536,864 | $3,577,440 | 433 | $93.8M | Research |
| Life Protect 24/7 INC | Administrative and Support and Waste Management | $2,681,300 | $2,712,731 | 423 | $80.0M | Research |
| People for the Ethical Treatment of Animals INC | Other Services (except Public Administration) | $2,603,800 | $1,927,484 | 219 | N/A | Research |
| Louise W. Eggleston Center INC | Health Care and Social Assistance | $2,409,800 | $1,962,086 | 349 | N/A | Research |
| Foundation to Support Animal Protection | Administrative and Support and Waste Management | $2,382,600 | $1,734,032 | 203 | N/A | Research |
| Vanguard Industries East, Inc. | Manufacturing | $2,349,800 | $2,375,778 | 208 | $73.4M | Research |
| Norfolk Tug Company | Transportation and Warehousing | $2,342,600 | $2,368,499 | 137 | $10.7M | Research |
| Atlantic Commtech Corporation | Information | $2,320,500 | $707,953 | 124 | $20.8M | Research |
| Auxiliary Systems INC | Manufacturing | $2,300,700 | $2,331,632 | 190 | $49.7M | Research |
| Tidewater Fleet Supply | Wholesale Trade | $2,172,550 | $2,196,388 | 182 | $55.8M | Research |
| Lyon Shipyard Inc. | Manufacturing | $2,000,000 | $2,012,889 | 295 | $77.2M | Research |
| Glasser and Glasser P.l.c. | Professional, Scientific, and Technical Services | $2,000,000 | $2,012,722 | 167 | $40.6M | Research |
| Vanguard Industries East Inc. | Manufacturing | $1,974,342 | $1,987,230 | 199 | $70.2M | Research |
| Hope House Foundation | Health Care and Social Assistance | $1,953,600 | $1,972,648 | 301 | N/A | Research |
| Glasser and Glasser, P.l.c. | Professional, Scientific, and Technical Services | $1,936,700 | $1,954,668 | 145 | $35.2M | Research |
| Vandeventer Black LLP | Professional, Scientific, and Technical Services | $1,936,100 | $1,957,343 | 109 | $26.5M | Research |