| Roanoke Fruit and Produce Company | Wholesale Trade | $151,300 | $152,788 | 18 | <$5M |  AI Deep Dive |
| Life Academy of the Roanoke Valley | Other Services (except Public Administration) | $151,100 | $152,514 | 47 | N/A | AI Deep Dive |
| Wayne Maxey | Manufacturing | $150,585 | $0 | 6 | $6.0M | AI Deep Dive |
| Heritage Home Care, INC | Health Care and Social Assistance | $150,300 | $151,729 | 46 | $7.9M | AI Deep Dive |
| Lcm Corporation | Administrative and Support and Waste Management | $150,000 | $151,867 | 15 | <$5M | AI Deep Dive |
| Schroeder Optical Co | Manufacturing | $149,900 | $150,371 | 19 | $18.8M | AI Deep Dive |
| Magee Goldstein Lasky & Sayers P.c. | Professional, Scientific, and Technical Services | $149,738 | $151,069 | 8 | <$5M | AI Deep Dive |
| Magee, Goldstein, Lasky and Sayers, PC | Professional, Scientific, and Technical Services | $149,700 | $151,355 | 8 | <$5M | AI Deep Dive |
| Jroc Holdings LLC | Other Services (except Public Administration) | $149,234 | $150,506 | 23 | <$5M | AI Deep Dive |
| The Hollins Group INC | Administrative and Support and Waste Management | $148,885 | $150,948 | 18 | <$5M | AI Deep Dive |
| Bloomfield Partners LLC | Professional, Scientific, and Technical Services | $148,587 | $149,070 | 10 | <$5M | AI Deep Dive |
| Bloomfield Partners LLC | Professional, Scientific, and Technical Services | $148,587 | $148,995 | 10 | <$5M | AI Deep Dive |
| Lucky Holdings LLC | Accommodation and Food Services | $148,500 | $149,865 | 20 | <$5M | AI Deep Dive |
| T.j.s. Enterprises Inc. | Administrative and Support and Waste Management | $148,200 | $149,645 | 19 | <$5M | AI Deep Dive |
| Canons Skye Corp | Retail Trade | $148,200 | $149,036 | 12 | <$5M | AI Deep Dive |
| Cardinal Tools, Sales & Service, Inc. | Wholesale Trade | $148,000 | $149,172 | 7 | <$5M | AI Deep Dive |
| Duncan Properties and Construction, Inc. | Construction | $147,887 | $149,212 | 12 | <$5M | AI Deep Dive |
| Paulamaral Inc. | Accommodation and Food Services | $147,805 | $148,704 | 24 | <$5M | AI Deep Dive |
| Toast of SW Roanoke LLC | Accommodation and Food Services | $146,500 | $147,912 | 30 | $5.3M | AI Deep Dive |
| D & S Life Agency Incorporated | Finance and Insurance | $146,100 | $147,381 | 10 | $5.1M | AI Deep Dive |
| American National University of Tennessee INC | Educational Services | $146,000 | $146,976 | 29 | <$5M | AI Deep Dive |
| Lasercam Inc. | Manufacturing | $145,875 | $147,106 | 22 | $5.8M | AI Deep Dive |
| Universal Rx LLC | Other Services (except Public Administration) | $145,600 | $146,886 | 11 | <$5M | AI Deep Dive |
| Gary A. Roach D.d.s. & Jonathan Lubeck D.m.d. Ltd. | Health Care and Social Assistance | $145,500 | $146,361 | 13 | <$5M | AI Deep Dive |
| Semtner & Semtner Dds, INC | Health Care and Social Assistance | $145,300 | $146,980 | 16 | <$5M | AI Deep Dive |