| A & L Environmental, Inc. | Administrative and Support and Waste Management | $39,677 | $40,061 | 8 | <$5M | AI Deep Dive |
| Business Data Systems | Information | $39,237 | $39,673 | 4 | <$5M | AI Deep Dive |
| B & B Fasteners, Inc. | Wholesale Trade | $39,000 | $39,235 | 4 | <$5M | AI Deep Dive |
| Eagle American LLC | Real Estate and Rental and Leasing | $38,200 | $38,546 | 3 | <$5M | AI Deep Dive |
| Titan Automation and Integrated Systems Inc. | Construction | $37,700 | $38,042 | 7 | <$5M | AI Deep Dive |
| Valley Skyline S Corporation | Accommodation and Food Services | $37,000 | $37,307 | 10 | <$5M | AI Deep Dive |
| Devkison LLC | Accommodation and Food Services | $37,000 | $37,252 | 12 | <$5M | AI Deep Dive |
| New Leaf Maintenance and Remodeling Inc. | Construction | $36,855 | $37,064 | 6 | <$5M | AI Deep Dive |
| Sharp Cars Ltd. | Retail Trade | $36,610 | $36,842 | 7 | <$5M | AI Deep Dive |
| Caroline A. Wallace, Dds, PC | Health Care and Social Assistance | $36,500 | $44,837 | 5 | <$5M | AI Deep Dive |
| Robyn Smith Ellis Plc | Professional, Scientific, and Technical Services | $36,100 | $36,345 | 5 | <$5M | AI Deep Dive |
| Tight Technologies INC | Information | $36,100 | $36,429 | 2 | <$5M | AI Deep Dive |
| Valley Podiatry | Health Care and Social Assistance | $35,700 | $35,973 | 6 | <$5M | AI Deep Dive |
| Tim Maiden Automotive, Inc. | Other Services (except Public Administration) | $35,643 | $35,940 | 4 | <$5M | AI Deep Dive |
| The Juilliard Corporation | Health Care and Social Assistance | $34,900 | $35,141 | 4 | <$5M | AI Deep Dive |
| Cavern Food INC | Accommodation and Food Services | $34,814 | $35,124 | 8 | <$5M | AI Deep Dive |
| Transmission Clinic LLC | Other Services (except Public Administration) | $34,392 | $34,914 | 6 | <$5M | AI Deep Dive |
| Benefit Service Integration, INC | Finance and Insurance | $34,253 | $34,425 | 4 | <$5M | AI Deep Dive |
| Valley Printers, Inc. | Administrative and Support and Waste Management | $34,228 | $34,607 | 5 | <$5M | AI Deep Dive |
| Valley Printers Inc. | Administrative and Support and Waste Management | $34,227 | $34,605 | 6 | <$5M | AI Deep Dive |
| 3 S Holdings, LLC | Retail Trade | $33,800 | $34,032 | 7 | <$5M | AI Deep Dive |
| Stephen Wade | Construction | $33,576 | $33,830 | 6 | <$5M | AI Deep Dive |
| Roanoke Valley Childrens Choir INC | Arts, Entertainment, and Recreation | $33,360 | $33,459 | 7 | N/A | AI Deep Dive |
| Dennis Carter | Manufacturing | $33,100 | $33,312 | 3 | <$5M | AI Deep Dive |
| College Evangelical Lutheran Church | Other Services (except Public Administration) | $32,800 | $33,059 | 5 | N/A | AI Deep Dive |