| Appalachian Trail Conservancy | Other Services (except Public Administration) | $875,783 | $880,283 | 57 | N/A |  Research |
| River Riders INC | Arts, Entertainment, and Recreation | $588,861 | $593,539 | 21 | <$5M | Research |
| Kinexum Services, LLC | Professional, Scientific, and Technical Services | $336,979 | $340,115 | 37 | <$5M | Research |
| River Riders, INC | Arts, Entertainment, and Recreation | $330,700 | $335,082 | 21 | <$5M | Research |
| Family Adventure Resort LLC | Accommodation and Food Services | $309,931 | $311,326 | 41 | $12.8M | Research |
| Family Adventure Resort, LLC | Accommodation and Food Services | $219,700 | $222,623 | 41 | $12.8M | Research |
| Through the Gardens INC | Administrative and Support and Waste Management | $165,000 | $155,912 | 19 | <$5M | Research |
| Dr Emily J Mayhew and Dr Edward R Smith | Health Care and Social Assistance | $160,712 | $161,895 | 12 | <$5M | Research |
| Dr Emily Mayhew and Dr Edward Smith INC | Health Care and Social Assistance | $160,700 | $162,378 | 12 | <$5M | Research |
| Harpers Ferry Fork and Fiddle LLC | Accommodation and Food Services | $149,043 | $150,364 | 9 | <$5M | Research |
| Harpers Fery Rok and Fiddle LLC | Retail Trade | $110,000 | $111,329 | 9 | <$5M | Research |
| Spencer Holding Company | Other Services (except Public Administration) | $89,940 | $79,898 | 10 | <$5M | Research |
| Sanfa Development INC | Accommodation and Food Services | $78,390 | $79,144 | 15 | <$5M | Research |
| Hill Impact LLC | Professional, Scientific, and Technical Services | $67,349 | $68,473 | 4 | <$5M | Research |
| Hillside Child Development Center | Health Care and Social Assistance | $62,600 | $62,908 | 19 | <$5M | Research |
| E3 Government Consulting LLC | Administrative and Support and Waste Management | $57,826 | $58,588 | 4 | <$5M | Research |
| Be-Lite for Life Medical Center | Unknown | $56,580 | $57,164 | 23 | N/A | Research |
| Sanfa Development INC | Accommodation and Food Services | $55,900 | $55,992 | 6 | <$5M | Research |
| Almost Heaven Pub and Grill LLC | Accommodation and Food Services | $47,597 | $48,096 | 4 | <$5M | Research |
| Stone Ridge Home Contracting LLC | Construction | $44,274 | $31,735 | 4 | <$5M | Research |
| Vivo Salon and Day Spa INC | Other Services (except Public Administration) | $42,000 | $42,537 | 4 | <$5M | Research |
| Almost Heaven Pub and Grill LLC | Accommodation and Food Services | $41,600 | $42,133 | 10 | <$5M | Research |
| Harpers Ferry Chiropractic and Nutrition Pllc | Health Care and Social Assistance | $40,268 | $40,478 | 5 | <$5M | Research |
| Harpers Ferry Chiropractic and Nutrition, Pllc | Health Care and Social Assistance | $40,200 | $40,615 | 5 | <$5M | Research |
| Preferred Maintenance Group Inc. | Administrative and Support and Waste Management | $39,971 | $40,422 | 13 | <$5M | Research |