| Ultimate Health Services, Inc. | Health Care and Social Assistance | $4,978,835 | $5,039,826 | 391 | $122.7M |  Research |
| J.h. Fletcher & Company | Manufacturing | $4,434,700 | $4,484,714 | 240 | $62.8M | Research |
| Valley Health Systems, Inc. | Health Care and Social Assistance | $3,998,232 | $4,041,879 | 279 | N/A | Research |
| Pritchard Electric Company | Construction | $3,945,600 | $3,996,126 | 215 | $70.7M | Research |
| Contractors Rental Corporation | Construction | $3,455,200 | $3,494,935 | 58 | $18.0M | Research |
| Cj Hughes Construction Company | Construction | $3,255,600 | $2,370,725 | 209 | $64.8M | Research |
| Marshall University Research Corporation | Professional, Scientific, and Technical Services | $3,115,000 | $2,589,429 | 362 | N/A | Research |
| Ohio Valley Physicians, Inc. | Health Care and Social Assistance | $3,024,760 | $3,064,613 | 227 | $71.2M | Research |
| Rubberlite, Inc. | Manufacturing | $2,500,000 | $2,528,992 | 151 | $149.8M | Research |
| Richwood Industries, Inc. | Manufacturing | $2,455,000 | $2,427,528 | 202 | $52.9M | Research |
| Manpower of Wv, Inc. | Administrative and Support and Waste Management | $2,404,273 | $2,434,193 | 475 | $74.8M | Research |
| Champion Industries | Manufacturing | $2,374,300 | $2,401,473 | 200 | $198.4M | Research |
| Allied Food Industries, Inc. | Accommodation and Food Services | $2,344,855 | $1,800,655 | 500 | $88.8M | Research |
| Hd Media, LLC | Information | $2,146,800 | $2,171,965 | 217 | $36.4M | Research |
| Dixon Electrical Systems & Contracting Inc. | Construction | $2,056,282 | $2,080,056 | 75 | $24.7M | Research |
| J. H. Fletcher & Co | Manufacturing | $2,000,000 | $2,034,167 | 203 | $53.2M | Research |
| Champion Industries | Manufacturing | $2,000,000 | $0 | 250 | $248.0M | Research |
| Autism Services Center, Inc. | Health Care and Social Assistance | $1,894,300 | $1,914,769 | 306 | N/A | Research |
| Hd Media Llc. | Information | $1,893,863 | $1,922,902 | 198 | $33.3M | Research |
| Dixon Electrical Systems & Contracting Inc. | Construction | $1,889,540 | $1,908,332 | 66 | $21.7M | Research |
| The C. I. Thornburg Co., Inc. | Construction | $1,729,328 | $1,740,604 | 95 | $29.4M | Research |
| Spriggs Distributing Co., Inc. - West Division | Wholesale Trade | $1,724,800 | $1,739,024 | 88 | $22.7M | Research |
| Radiology Inc. | Health Care and Social Assistance | $1,720,605 | $1,734,464 | 58 | $18.2M | Research |
| Restaurant Enterprises, Inc. | Accommodation and Food Services | $1,583,655 | $1,481,278 | 421 | $74.8M | Research |
| Radiology, Inc. | Health Care and Social Assistance | $1,542,050 | $1,558,273 | 61 | $19.1M | Research |