| Preston Contractors, Inc. | Construction | $4,576,623 | $4,630,163 | 192 | $71.9M |  Research |
| Preston Memorial Hospital Corporation | Health Care and Social Assistance | $3,404,920 | $3,444,007 | 440 | N/A | Research |
| Mountaineer Contractors, Inc. | Construction | $1,736,923 | $1,757,290 | 40 | $12.4M | Research |
| Rock Forge Bridge Co LLC | Construction | $1,115,641 | $1,127,959 | 64 | $19.8M | Research |
| Gulfstream West Investments, Inc. | Management of Companies and Enterprises | $599,275 | $605,941 | 31 | $5.1M | Research |
| The Auto Source of West Virginia, Inc. | Retail Trade | $377,292 | $382,481 | 47 | $11.8M | Research |
| West Virginia Military Support Services Inc. | Other Services (except Public Administration) | $375,043 | $377,601 | 53 | N/A | Research |
| West Virginia Military Support Services, Inc. | Other Services (except Public Administration) | $371,900 | $173,960 | 111 | N/A | Research |
| Preston County Senior Citizens INC | Health Care and Social Assistance | $281,150 | $230,357 | 148 | N/A | Research |
| Maloney & Associates, P.l.l.c. | Professional, Scientific, and Technical Services | $232,398 | $234,136 | 15 | <$5M | Research |
| Hillside Trucking INC | Transportation and Warehousing | $211,277 | $213,337 | 18 | <$5M | Research |
| Hillside Trucking Inc. | Transportation and Warehousing | $211,277 | $213,233 | 21 | <$5M | Research |
| Victory Auto Group LLC | Retail Trade | $179,132 | $180,421 | 17 | <$5M | Research |
| Preston Rehabilitation and Orthopedic Physical Therapy, Inc. | Health Care and Social Assistance | $162,000 | $163,407 | 15 | <$5M | Research |
| Victory Auto Group LLC | Retail Trade | $161,600 | $163,656 | 18 | <$5M | Research |
| Kittanning Contractors, LLC | Construction | $150,159 | $151,830 | 20 | $7.5M | Research |
| Monroes Deli Style Eateryllc | Accommodation and Food Services | $126,975 | $128,140 | 17 | <$5M | Research |
| Windy Hill Manor Limited Partnership | Health Care and Social Assistance | $125,747 | $127,004 | 25 | <$5M | Research |
| Preston Urgent Care/family Practice LLC | Health Care and Social Assistance | $122,000 | $123,146 | 10 | <$5M | Research |
| Preston Urgent Care Family/practice LLC | Health Care and Social Assistance | $112,003 | $112,582 | 15 | <$5M | Research |
| Phil Graham LLC | Accommodation and Food Services | $110,964 | $112,457 | 30 | $5.3M | Research |
| Schwarzenberg & Schwarzenberg Mds | Health Care and Social Assistance | $100,000 | $90,247 | 11 | <$5M | Research |
| Preston Silver Eagle Co. | Wholesale Trade | $96,800 | $97,731 | 8 | <$5M | Research |
| Surveyor and Associates, Inc. | Professional, Scientific, and Technical Services | $95,057 | $96,070 | 13 | <$5M | Research |
| Ali Co. | Construction | $92,088 | $92,799 | 11 | <$5M | Research |