| Matheny Motor Truck Company | Retail Trade | $4,062,500 | $4,109,670 | 250 | $63.0M |  Research |
| United Construction Company INC | Manufacturing | $3,596,400 | $3,637,759 | 130 | $31.8M | Research |
| Wincore Window Company LLC | Manufacturing | $2,786,700 | $2,819,599 | 500 | $495.9M | Research |
| Westbrook Health Services, Inc. | Health Care and Social Assistance | $2,429,600 | $2,457,810 | 454 | N/A | Research |
| Hg Energy LLC | Mining, Quarrying, and Oil and Gas Extraction | $1,677,465 | $1,691,817 | 74 | <$5M | Research |
| Hg Energy LLC | Mining, Quarrying, and Oil and Gas Extraction | $1,558,500 | $1,568,024 | 70 | <$5M | Research |
| First Settlement Physical Therapy INC | Health Care and Social Assistance | $1,371,973 | $1,384,039 | 143 | $26.1M | Research |
| First Settlement Physical Therapy INC | Health Care and Social Assistance | $1,371,973 | $1,383,062 | 130 | $35.1M | Research |
| Mountain River Physical Therapy, L.l.c. | Health Care and Social Assistance | $1,295,100 | $1,210,336 | 123 | $33.2M | Research |
| Murray's Sheet Metal Company, | Manufacturing | $1,246,700 | $1,256,611 | 63 | $15.4M | Research |
| Pickering Associates INC | Professional, Scientific, and Technical Services | $1,215,446 | $1,223,043 | 56 | $6.9M | Research |
| North Parkersburg Baptist Church | Other Services (except Public Administration) | $1,207,122 | $222,560 | 41 | $7.8M | Research |
| Jan Dils Attorney at Law, Lc | Professional, Scientific, and Technical Services | $1,053,104 | $1,061,702 | 123 | $29.9M | Research |
| Pickering Associates, INC | Professional, Scientific, and Technical Services | $993,200 | $999,904 | 64 | $7.9M | Research |
| T. W. J., Inc. | Retail Trade | $981,077 | $989,490 | 249 | $62.2M | Research |
| Cfi Engineering Company | Administrative and Support and Waste Management | $871,982 | $880,869 | 88 | $13.8M | Research |
| Parkersburg Cardiology Associates INC | Health Care and Social Assistance | $866,200 | $873,154 | 56 | $17.6M | Research |
| Astorg Imports INC | Retail Trade | $805,800 | $816,052 | 63 | $15.9M | Research |
| Astorg Ford of Parkersburg, Inc. | Retail Trade | $776,400 | $786,278 | 76 | $19.2M | Research |
| Architectural Interior Products INC | Wholesale Trade | $756,930 | $761,535 | 58 | $17.8M | Research |
| Architectural Interior Products, INC | Retail Trade | $756,900 | $761,754 | 71 | $14.7M | Research |
| Louis Thomas Subaru, Inc. | Retail Trade | $754,200 | $763,083 | 25 | $6.3M | Research |
| Grogg's Heating and Air Conditioning, INC | Construction | $717,977 | $724,271 | 53 | $16.8M | Research |
| Delton Restaurants, Inc. | Accommodation and Food Services | $671,600 | $677,103 | 206 | $36.6M | Research |
| United Anesthesia, INC | Health Care and Social Assistance | $670,600 | $676,747 | 29 | $9.1M | Research |