| Alderson Broaddus University | Educational Services | $1,998,200 | $2,001,569 | 228 | $27.4M |  Research |
| P & P Foods, Inc. | Retail Trade | $402,697 | $406,444 | 79 | $19.7M | Research |
| Mace's Pharmacy, Inc. | Retail Trade | $331,201 | $334,633 | 29 | $5.1M | Research |
| Midland Resource Recovery Inc. | Professional, Scientific, and Technical Services | $278,093 | $281,406 | 14 | <$5M | Research |
| Midland Resource Recovery INC | Mining, Quarrying, and Oil and Gas Extraction | $261,210 | $263,728 | 14 | <$5M | Research |
| Agvets INC | Professional, Scientific, and Technical Services | $258,500 | $261,028 | 25 | <$5M | Research |
| Key Oil Company | Mining, Quarrying, and Oil and Gas Extraction | $196,873 | $199,317 | 13 | <$5M | Research |
| Key Oil Company | Mining, Quarrying, and Oil and Gas Extraction | $196,800 | $197,970 | 13 | <$5M | Research |
| Barbour County Senior Center, Inc. | Health Care and Social Assistance | $183,315 | $185,698 | 60 | N/A | Research |
| Brj Management LLC | Real Estate and Rental and Leasing | $133,557 | $134,752 | 7 | <$5M | Research |
| Barbour County Emergency Squad INC | Health Care and Social Assistance | $131,500 | $132,271 | 37 | $8.6M | Research |
| Barbour County Emergency Squad INC | Health Care and Social Assistance | $131,500 | $132,789 | 30 | $7.0M | Research |
| Mullens & Mullens Pllc | Professional, Scientific, and Technical Services | $93,459 | $94,282 | 7 | <$5M | Research |
| Mullens & Mullens, Pllc | Professional, Scientific, and Technical Services | $81,702 | $82,403 | 7 | <$5M | Research |
| SW Group LLC | Construction | $75,000 | $75,548 | 5 | $5.7M | Research |
| Tygart Valley Trucking & Excavating Company LLC | Mining, Quarrying, and Oil and Gas Extraction | $57,256 | $58,434 | 7 | <$5M | Research |
| Tygart Valley Trucking & Excavating Company LLC | Mining, Quarrying, and Oil and Gas Extraction | $53,141 | $53,795 | 11 | <$5M | Research |
| Extreme Endeavors and Consulting | Professional, Scientific, and Technical Services | $48,100 | $48,445 | 6 | <$5M | Research |
| Little Moe's INC | Accommodation and Food Services | $47,400 | $47,831 | 9 | <$5M | Research |
| Heart and Hand House, Inc. | Retail Trade | $47,397 | $47,704 | 12 | N/A | Research |
| Extreme Endeavors and Consulting LLC | Manufacturing | $43,900 | $35,894 | 8 | <$5M | Research |
| Medallion, Inc. | Accommodation and Food Services | $37,347 | $20,712 | 13 | <$5M | Research |
| Little Moes INC | Accommodation and Food Services | $33,800 | $34,003 | 9 | <$5M | Research |
| Daycon, INC | Construction | $33,400 | $33,633 | 8 | $9.1M | Research |
| Cvechko Insurance Services | Finance and Insurance | $33,100 | $33,328 | 3 | <$5M | Research |