| Perry & Associates Certified Public Accountants Ac | Professional, Scientific, and Technical Services | $896,800 | $905,031 | 65 | $20.7M |  Research |
| Ohio Valley University, INC | Educational Services | $712,100 | $718,410 | 173 | N/A | Research |
| Ohio Valley University Inc. | Educational Services | $585,340 | $592,380 | 170 | N/A | Research |
| Neale Marine Transportation & Fleeting Service, LLC | Transportation and Warehousing | $363,455 | $366,074 | 26 | $6.6M | Research |
| Valeo Medical Pllc | Health Care and Social Assistance | $307,015 | $309,420 | 36 | $11.3M | Research |
| The Pm Company | Real Estate and Rental and Leasing | $260,100 | $263,424 | 25 | <$5M | Research |
| Valeo Medical Pllc | Health Care and Social Assistance | $259,590 | $261,075 | 31 | $5.5M | Research |
| Parkersburg Surgical Associates Pllc | Health Care and Social Assistance | $231,900 | $234,097 | 15 | <$5M | Research |
| Alliance Xpress Care LLC | Health Care and Social Assistance | $231,748 | $232,913 | 30 | $9.4M | Research |
| Tenney & Associates, Ll | Professional, Scientific, and Technical Services | $203,830 | $205,214 | 14 | <$5M | Research |
| Gregory E Krause MD INC | Health Care and Social Assistance | $200,370 | $201,912 | 14 | <$5M | Research |
| Pretty Nails 1 LLC | Other Services (except Public Administration) | $200,000 | $202,261 | 21 | <$5M | Research |
| Pretty Nails 1 LLC | Other Services (except Public Administration) | $200,000 | $202,383 | 27 | $5.2M | Research |
| Lamp Pest Control, INC | Administrative and Support and Waste Management | $191,200 | $193,510 | 22 | <$5M | Research |
| Plaza Maya INC | Accommodation and Food Services | $172,843 | $174,053 | 23 | <$5M | Research |
| Las Trancas of Charleston INC | Accommodation and Food Services | $164,729 | $165,924 | 21 | <$5M | Research |
| Pleasant Air Heating and Cooling INC | Construction | $158,600 | $160,107 | 16 | $5.1M | Research |
| Gregory E Krause MD | Health Care and Social Assistance | $155,623 | $156,163 | 13 | <$5M | Research |
| Habitat for Humanity of the Mid-Ohio Valley | Other Services (except Public Administration) | $154,400 | $155,871 | 17 | <$5M | Research |
| H. Talbott Tebay, D.d.s., Ltd. | Health Care and Social Assistance | $153,000 | $154,641 | 15 | <$5M | Research |
| T.m.t. 2 Enterprises | Accommodation and Food Services | $150,000 | $151,454 | 45 | $8.0M | Research |
| Rbs Healthcare, LLC | Health Care and Social Assistance | $149,600 | $151,586 | 30 | $5.1M | Research |
| Las Trancas of Ripley INC | Accommodation and Food Services | $144,335 | $145,297 | 18 | <$5M | Research |
| R. Joe Weaver, O.d. | Health Care and Social Assistance | $136,233 | $137,192 | 16 | <$5M | Research |
| Jimmie Harper Construction | Construction | $125,729 | $127,172 | 17 | $5.8M | Research |