| Environmental Reclamation Solutions LLC | Construction | $2,039,000 | $812,468 | 46 | $17.2M |  Research |
| Productive Living Systems, Inc. | Health Care and Social Assistance | $1,301,400 | $0 | 227 | $38.6M | Research |
| Nbi, INC | Educational Services | $1,200,000 | $1,210,933 | 95 | N/A | Research |
| Chippewa Valley Orthopedics & Sports Medicine, Sc | Health Care and Social Assistance | $1,199,700 | $1,210,481 | 73 | $22.9M | Research |
| Certified Inc. | Construction | $1,088,634 | $1,092,989 | 53 | $16.8M | Research |
| Certified, Inc. | Construction | $1,088,600 | $1,096,220 | 50 | $15.8M | Research |
| Benedict Refrigeration Service INC | Construction | $1,054,200 | $1,061,236 | 81 | $30.3M | Research |
| Oral and Maxillofacial Surgery of Eau Claire Sc | Health Care and Social Assistance | $631,600 | $635,355 | 49 | $11.4M | Research |
| Nsh Altoona LLC | Health Care and Social Assistance | $520,700 | $533,140 | 130 | $22.1M | Research |
| Hoeft Builders INC | Construction | $493,500 | $496,610 | 33 | $37.6M | Research |
| Senasys, Inc. | Manufacturing | $479,020 | $482,708 | 64 | $16.8M | Research |
| The Classic at Hillcrest Greens LLC | Health Care and Social Assistance | $385,600 | $390,819 | 120 | $20.4M | Research |
| Clear Water Brands Inc. | Information | $385,225 | $390,006 | 32 | $5.4M | Research |
| Eau Claire Golf and Country Club | Arts, Entertainment, and Recreation | $369,900 | $371,380 | 152 | N/A | Research |
| Premium Lawn Service, LLC | Administrative and Support and Waste Management | $364,094 | $366,208 | 45 | $9.7M | Research |
| Northwoods Sport & Hand, Inc. | Health Care and Social Assistance | $355,700 | $359,079 | 25 | $6.8M | Research |
| Clear Water Brands, Inc. | Information | $349,879 | $388,465 | 37 | $6.2M | Research |
| Dimension X Design, LLC | Professional, Scientific, and Technical Services | $345,500 | $347,602 | 16 | <$5M | Research |
| Shillin Wealth Management LLC | Finance and Insurance | $331,800 | $0 | 14 | $7.2M | Research |
| Hagen Decorators, Inc. | Construction | $323,400 | $327,047 | 25 | $8.2M | Research |
| Bernicke Wealth Management Ltd. | Finance and Insurance | $320,900 | $323,239 | 24 | $12.3M | Research |
| Vs Sports Bar INC | Accommodation and Food Services | $254,743 | $256,646 | 50 | $8.9M | Research |
| Premium Lawn Service, LLC | Professional, Scientific, and Technical Services | $253,810 | $255,474 | 41 | $8.3M | Research |
| Johnson Runkel & Kraegenbrink, Inc. | Professional, Scientific, and Technical Services | $246,900 | $250,267 | 10 | <$5M | Research |
| Adam Mohr | Finance and Insurance | $239,100 | $72,983 | 15 | $7.7M | Research |