| Heart and Vascular Institute of Wisconsin, S.c. | Health Care and Social Assistance | $1,586,600 | $1,604,944 | 108 | $33.9M |  Research |
| Partnership Community Health Center | Health Care and Social Assistance | $1,549,232 | $1,571,309 | 125 | $39.2M | Research |
| Surface Mount Technology Corporation | Professional, Scientific, and Technical Services | $1,544,300 | $1,552,085 | 155 | $16.0M | Research |
| Flex-Staff, Inc. | Administrative and Support and Waste Management | $1,509,900 | $1,522,517 | 240 | $37.8M | Research |
| R. Sabee Company, LLC | Manufacturing | $1,500,000 | $1,517,260 | 140 | $138.9M | Research |
| Rochelle Leigh Group LLC | Other Services (except Public Administration) | $1,489,715 | $1,501,468 | 190 | $36.3M | Research |
| Skilltech, Inc. | Other Services (except Public Administration) | $1,484,000 | $1,492,863 | 91 | $19.6M | Research |
| Community Blood Center, Inc. | Health Care and Social Assistance | $1,456,139 | $1,466,472 | 149 | N/A | Research |
| Hand to Shoulder Center of Wisconsin | Health Care and Social Assistance | $1,444,300 | $1,454,370 | 110 | $19.6M | Research |
| Alliance Industries, Inc. | Manufacturing | $1,435,700 | $1,444,474 | 140 | $34.2M | Research |
| Advanced Physical Therapy & Sports Medicine Sc | Health Care and Social Assistance | $1,414,945 | $1,423,202 | 99 | $26.7M | Research |
| Advanced Physical Therapy & Sports Medicine, Sc | Health Care and Social Assistance | $1,414,900 | $1,423,893 | 99 | $26.7M | Research |
| Association of Hospital Anesthesiologists | Health Care and Social Assistance | $1,407,200 | $1,422,583 | 47 | $14.8M | Research |
| The Barber Shop LLC | Other Services (except Public Administration) | $1,391,000 | $1,506,337 | 234 | $44.7M | Research |
| Aia Holding Company | Professional, Scientific, and Technical Services | $1,376,500 | $1,389,699 | 103 | $11.5M | Research |
| Women's Care of Wisconsin, S.c. | Health Care and Social Assistance | $1,363,100 | $1,370,569 | 95 | $29.8M | Research |
| Limitless Possibilities LLC | Health Care and Social Assistance | $1,306,900 | $1,317,176 | 238 | $40.5M | Research |
| Pro Label INC | Manufacturing | $1,301,400 | $1,310,100 | 144 | $142.8M | Research |
| Hoffman Planning Design & | Professional, Scientific, and Technical Services | $1,249,838 | $1,259,177 | 56 | $17.4M | Research |
| Hoffman Planning, Design & Construction, Inc. | Professional, Scientific, and Technical Services | $1,249,800 | $1,257,577 | 56 | $17.4M | Research |
| Aia Holding Company | Professional, Scientific, and Technical Services | $1,172,578 | $1,185,685 | 71 | $8.0M | Research |
| Security-Luebke Roofing, Inc. | Construction | $1,125,000 | $1,131,281 | 96 | $22.2M | Research |
| B & H Pattern Inc. | Manufacturing | $1,090,800 | $1,104,039 | 77 | $18.8M | Research |
| B & H Pattern Inc. | Manufacturing | $1,090,700 | $1,101,607 | 74 | $18.1M | Research |
| Stumpf Motor Co., Inc. | Retail Trade | $1,068,800 | $1,075,564 | 118 | $29.7M | Research |