| Prairie Ridge Health INC | Wholesale Trade | $4,180,540 | $4,229,676 | 387 | $118.6M |  Research |
| Columbus Chemical Industries, INC | Wholesale Trade | $941,889 | $950,689 | 70 | $18.0M | Research |
| G. D. Roberts and Co., Inc. | Manufacturing | $911,808 | $921,950 | 76 | $18.6M | Research |
| Gar Plastics, Inc. | Manufacturing | $545,900 | $548,831 | 45 | $44.6M | Research |
| Gar Plastics Inc. | Manufacturing | $514,202 | $516,146 | 81 | $80.3M | Research |
| Stakes INC | Accommodation and Food Services | $497,157 | $501,625 | 150 | $26.6M | Research |
| Countryside Ford, Inc. | Retail Trade | $411,200 | $414,647 | 27 | $6.8M | Research |
| Columbus Family Dental | Health Care and Social Assistance | $400,088 | $402,554 | 34 | $7.9M | Research |
| Columbus Family Dental INC | Health Care and Social Assistance | $385,237 | $387,021 | 37 | $8.6M | Research |
| Stakes INC | Accommodation and Food Services | $355,112 | $95,988 | 150 | $26.6M | Research |
| Duffy Bros. Inc. | Wholesale Trade | $341,500 | $343,081 | 21 | $5.4M | Research |
| Duffy Bros., Inc. | Wholesale Trade | $272,500 | $274,478 | 28 | $7.2M | Research |
| Dexa Solutions LLC | Other Services (except Public Administration) | $233,824 | $235,104 | 18 | <$5M | Research |
| Dexa Solutions LLC | Professional, Scientific, and Technical Services | $223,372 | $224,743 | 20 | <$5M | Research |
| Napleton Autowerks Wisconsin, INC | Retail Trade | $194,072 | $196,529 | 19 | <$5M | Research |
| Yawney Cabinets LLC | Manufacturing | $190,712 | $166,765 | 14 | <$5M | Research |
| Duffy Fleet Services INC | Other Services (except Public Administration) | $189,500 | $190,310 | 13 | <$5M | Research |
| Columbus Countryside Veterinary Clinic LLC | Professional, Scientific, and Technical Services | $175,100 | $177,302 | 31 | <$5M | Research |
| Duffy Fleet Services, Inc. | Accommodation and Food Services | $169,500 | $170,977 | 19 | <$5M | Research |
| Advantage Management Waupun LLC | Health Care and Social Assistance | $162,322 | $163,562 | 26 | <$5M | Research |
| Great Lakes Baptist Conference INC | Other Services (except Public Administration) | $150,900 | $151,801 | 17 | N/A | Research |
| St Jeromes Parish | Other Services (except Public Administration) | $142,777 | $143,927 | 29 | N/A | Research |
| Dnd Family Dairy, LLC | Agriculture, Forestry, Fishing and Hunting | $140,852 | $184,526 | 24 | <$5M | Research |
| R & G Miller & Sons INC | Agriculture, Forestry, Fishing and Hunting | $139,803 | $140,793 | 14 | <$5M | Research |
| Great Lakes Baptist Conference Incorporated | Other Services (except Public Administration) | $136,386 | $137,481 | 14 | N/A | Research |