| Lafayette County | Health Care and Social Assistance | $1,952,260 | $1,971,936 | 124 | N/A |  Research |
| Paramount Calves, LLC | Agriculture, Forestry, Fishing and Hunting | $425,400 | $427,824 | 32 | $5.2M | Research |
| Darlington Ridge Farm LLC | Agriculture, Forestry, Fishing and Hunting | $344,282 | $347,433 | 43 | $7.0M | Research |
| Darlington Ridge Farm, LLC | Agriculture, Forestry, Fishing and Hunting | $333,300 | $335,161 | 41 | $6.7M | Research |
| Darlington Dairy Supply Co., INC | Wholesale Trade | $269,145 | $271,702 | 22 | $6.7M | Research |
| Darlington Dairy Supply Co. | Manufacturing | $249,062 | $250,121 | 18 | <$5M | Research |
| Virtue's Auto Tech Inc. | Retail Trade | $171,000 | $171,960 | 19 | <$5M | Research |
| Virtue's Auto Tech Inc. | Retail Trade | $162,178 | $163,079 | 14 | <$5M | Research |
| See-Mor Grain, INC | Wholesale Trade | $156,110 | $157,030 | 8 | <$5M | Research |
| Dempsey Educational Services, LLC | Educational Services | $151,600 | $15,198 | 1 | <$5M | Research |
| Reilly Plumbing & Heating INC | Construction | $150,800 | $152,002 | 13 | <$5M | Research |
| K & L Bobcat, Inc. | Other Services (except Public Administration) | $119,695 | $120,789 | 10 | <$5M | Research |
| Tuescher Electric and Refigeration, Inc. | Construction | $119,505 | $120,302 | 10 | <$5M | Research |
| Stephen Carpenter | Agriculture, Forestry, Fishing and Hunting | $99,250 | $99,930 | 10 | <$5M | Research |
| Highway Dairy Farms LLC | Agriculture, Forestry, Fishing and Hunting | $98,100 | $99,133 | 15 | <$5M | Research |
| Bergeson Ventures Mineral Point LLC | Accommodation and Food Services | $97,839 | $98,367 | 33 | $5.9M | Research |
| Mathys Hardware, Inc. | Retail Trade | $94,359 | $94,943 | 13 | <$5M | Research |
| Schilling Farms LLC | Agriculture, Forestry, Fishing and Hunting | $93,700 | $94,167 | 11 | <$5M | Research |
| Nyff Trucking, LLC | Transportation and Warehousing | $92,750 | $93,388 | 11 | <$5M | Research |
| Nyff Trucking LLC | Transportation and Warehousing | $92,587 | $93,026 | 5 | <$5M | Research |
| Center Hill Veterinary Clinic, S.c. | Professional, Scientific, and Technical Services | $86,128 | $86,903 | 9 | <$5M | Research |
| Silver Creek Family Farms, LLC | Agriculture, Forestry, Fishing and Hunting | $82,900 | $83,441 | 10 | <$5M | Research |
| S.m.g. Insurance Services, Inc. | Finance and Insurance | $74,777 | $75,312 | 5 | <$5M | Research |
| Thunderbranch Acres Inc. | Agriculture, Forestry, Fishing and Hunting | $71,833 | $72,643 | 11 | <$5M | Research |
| Bergeson Ventures Mineral Point LLC | Accommodation and Food Services | $69,800 | $70,330 | 36 | $6.4M | Research |