| Big Radio | Information | $348,257 | $351,005 | 22 | <$5M | AI Deep Dive |
| Van Brocklin Electric, LLC | Construction | $344,600 | $346,488 | 25 | $8.2M | AI Deep Dive |
| Budget Truck and Auto Body INC | Other Services (except Public Administration) | $333,765 | $338,182 | 40 | $7.8M | AI Deep Dive |
| Gallina USA LLC | Manufacturing | $332,500 | $335,561 | 39 | $38.7M | AI Deep Dive |
| J.n.b. Signs Inc. | Manufacturing | $329,040 | $330,960 | 32 | $8.9M | AI Deep Dive |
| Budget Truck and Auto Body, Inc. | Other Services (except Public Administration) | $325,000 | $327,466 | 37 | $7.3M | AI Deep Dive |
| Lowell C. Hagen Trucking Inc. | Transportation and Warehousing | $324,100 | $327,092 | 42 | $11.2M | AI Deep Dive |
| Janesville Acquisition, Inc. | Retail Trade | $322,628 | $324,447 | 36 | $9.4M | AI Deep Dive |
| Home Run Auto Group, LLC | Retail Trade | $315,757 | $319,388 | 21 | $5.3M | AI Deep Dive |
| Premier Oral & Maxillofacial Surgery S.c. | Health Care and Social Assistance | $315,689 | $317,471 | 32 | $7.4M | AI Deep Dive |
| Ben Thompson | Information | $309,945 | $311,090 | 22 | <$5M | AI Deep Dive |
| J.n.b. Signs, INC | Manufacturing | $309,797 | $311,809 | 32 | $8.9M | AI Deep Dive |
| Janesville Autos, LLC | Retail Trade | $304,300 | $306,227 | 35 | $8.8M | AI Deep Dive |
| The Bloomin Apple Janesville LLC | Accommodation and Food Services | $301,430 | $304,139 | 48 | $8.5M | AI Deep Dive |
| Saalsaa Group Two Inc. | Accommodation and Food Services | $293,258 | $294,760 | 57 | $10.1M | AI Deep Dive |
| Harris Ace Hardware, LLP | Retail Trade | $287,820 | $289,705 | 46 | $9.5M | AI Deep Dive |
| Lions Quick Marts, Inc. | Retail Trade | $287,200 | $288,782 | 55 | $18.4M | AI Deep Dive |
| N1 Critical Technologies INC | Manufacturing | $282,977 | $283,993 | 11 | <$5M | AI Deep Dive |
| Janesville Travel Center Inc. | Retail Trade | $282,727 | $283,834 | 37 | $12.4M | AI Deep Dive |
| Ikon Transportation Services, Inc. | Transportation and Warehousing | $282,500 | $284,226 | 22 | <$5M | AI Deep Dive |
| Fontana Flavors Inc. | Manufacturing | $282,400 | $284,357 | 25 | $8.8M | AI Deep Dive |
| Wisconsin Fuel LLC | Retail Trade | $275,407 | $278,834 | 195 | $65.3M | AI Deep Dive |
| Wisconsin Fuel LLC | Manufacturing | $275,400 | $279,149 | 57 | $56.5M | AI Deep Dive |
| The Schlueter Company | Wholesale Trade | $270,100 | $271,691 | 25 | $7.7M | AI Deep Dive |
| Janesville Travel Center Inc. | Retail Trade | $269,800 | $271,508 | 36 | $12.0M | AI Deep Dive |