| Rottinghaus Company, Incorporated | Accommodation and Food Services | $9,466,800 | $9,549,372 | 500 | $88.8M |  Research |
| Inland Label and Marketing Services LLC | Manufacturing | $4,620,500 | $4,673,288 | 357 | $354.1M | Research |
| Fortney Hospitality Group, Inc. | Management of Companies and Enterprises | $3,942,600 | $3,955,367 | 500 | $82.2M | Research |
| Fowler & Hammer, INC | Construction | $2,965,000 | $2,999,199 | 158 | $180.0M | Research |
| Duratech Industries, Inc. | Manufacturing | $2,801,000 | $2,834,075 | 280 | $277.7M | Research |
| River States Truck and Trailer, Inc. | Retail Trade | $2,225,300 | $2,251,394 | 184 | $46.4M | Research |
| Fortney Hospitality Group Inc. | Accommodation and Food Services | $2,000,000 | $2,018,556 | 73 | $8.9M | Research |
| River States Truck and Trailer Inc. | Wholesale Trade | $1,950,000 | $1,962,875 | 184 | $56.4M | Research |
| N.a. | Professional, Scientific, and Technical Services | $1,865,900 | $1,883,885 | 169 | $53.8M | Research |
| Bethany Lutheran Homes, INC | Health Care and Social Assistance | $1,700,000 | $1,708,477 | 319 | N/A | Research |
| Dahl Automotive La Crosse, Inc. | Retail Trade | $1,692,200 | $1,705,320 | 142 | $35.8M | Research |
| Badger Corrugating Company | Wholesale Trade | $1,589,260 | $1,598,178 | 210 | $64.3M | Research |
| Kish & Sons Electric, Inc. | Construction | $1,578,700 | $1,590,453 | 90 | $29.6M | Research |
| Potato King Transportation | Transportation and Warehousing | $1,505,200 | $1,514,025 | 105 | $15.9M | Research |
| Aptiv, INC | Health Care and Social Assistance | $1,465,300 | $1,474,453 | 168 | N/A | Research |
| Aptiv INC | Health Care and Social Assistance | $1,327,365 | $1,332,493 | 271 | N/A | Research |
| La Crosse Area Family Young Men's Christian Association Inc. | Other Services (except Public Administration) | $1,317,115 | $1,332,628 | 481 | $91.9M | Research |
| Chileda Institute INC | Educational Services | $1,300,000 | $1,316,792 | 229 | N/A | Research |
| Fiberpro INC | Manufacturing | $1,207,205 | $1,215,555 | 135 | $133.9M | Research |
| Torrance Casting INC | Manufacturing | $1,194,600 | $1,207,462 | 101 | $26.4M | Research |
| Borton Construction, Inc. | Construction | $1,155,334 | $1,162,202 | 49 | $55.8M | Research |
| Earl L Bonsack INC | Transportation and Warehousing | $1,120,445 | $1,125,387 | 80 | $16.1M | Research |
| Mid-City Steel LLC | Manufacturing | $1,100,000 | $1,107,625 | 82 | $20.0M | Research |
| Pischke Motors of Lacrosse Inc. | Retail Trade | $1,081,500 | $1,089,204 | 60 | $15.1M | Research |
| Borton Construction Inc. | Construction | $1,077,099 | $1,083,502 | 79 | $90.0M | Research |