| Von Briesen & Roper S.c. | Professional, Scientific, and Technical Services | $10,000,000 | $7,698,488 | 340 | $82.6M | Research |
| J. M. Brennan, Inc. | Construction | $8,636,300 | $8,737,537 | 364 | $115.2M | Research |
| Derse Inc. | Manufacturing | $7,812,500 | $7,912,243 | 488 | $136.3M | Research |
| Corporate Group, Inc. | Construction | $7,642,000 | $7,732,029 | 481 | $158.3M | Research |
| Godfrey & Kahn, S.c. | Professional, Scientific, and Technical Services | $7,289,500 | $7,375,776 | 335 | $81.4M | Research |
| Hatco Corporation | Manufacturing | $6,563,062 | $6,640,958 | 500 | $130.9M | Research |
| Jacobus Energy, LLC | Wholesale Trade | $6,487,500 | $6,561,440 | 411 | $105.8M | Research |
| Dental Associates Family & Specialty Care LLC | Health Care and Social Assistance | $6,406,055 | $6,482,928 | 500 | $115.9M | Research |
| W T Walker Group INC | Manufacturing | $5,752,567 | $5,819,434 | 382 | $93.4M | Research |
| Auto Transtech, Inc. | Manufacturing | $5,645,800 | $5,722,521 | 500 | $130.9M | Research |
| Brodersen Management Corp | Accommodation and Food Services | $5,456,000 | $5,539,659 | 500 | $93.3M | Research |
| Butters-Fetting Co., Inc. | Construction | $5,150,500 | $5,209,343 | 306 | $100.7M | Research |
| Bader Rutter & Associates, Inc. | Professional, Scientific, and Technical Services | $5,098,900 | $5,157,572 | 289 | $32.4M | Research |
| Zilber Ltd. | Construction | $5,034,250 | $5,091,627 | 269 | $81.7M | Research |
| Perlick Corporation | Manufacturing | $4,979,200 | $5,042,770 | 318 | $83.3M | Research |
| Aids Resource Center of Wisconsin INC | Public Administration | $4,978,500 | $5,035,923 | 415 | N/A | Research |
| Kriete Group, LLC | Wholesale Trade | $4,833,100 | $4,888,714 | 390 | $119.5M | Research |
| Graef-Usa Inc. | Professional, Scientific, and Technical Services | $4,631,500 | $4,685,048 | 271 | $33.4M | Research |
| Paper Machinery Holding Corporation | Manufacturing | $4,622,860 | $4,675,589 | 235 | $61.5M | Research |
| Eppstein Uhen Architects, Inc. | Professional, Scientific, and Technical Services | $4,590,500 | $4,641,436 | 229 | $71.0M | Research |
| Lakeside International, LLC | Real Estate and Rental and Leasing | $4,560,000 | $4,613,845 | 323 | $146.0M | Research |
| Alverno College | Educational Services | $4,537,000 | $4,593,557 | 442 | N/A | Research |
| Sixteenth Street Community Health Centers, Inc. | Health Care and Social Assistance | $4,377,500 | $4,427,963 | 397 | N/A | Research |
| Sainta | Health Care and Social Assistance | $4,375,870 | $1,318,298 | 325 | N/A | Research |
| Mec International Corporation | Manufacturing | $4,244,200 | $4,294,665 | 342 | $89.5M | Research |