| Courtesy Corporation | Accommodation and Food Services | $10,000,000 | $10,133,973 | 500 | $88.8M |  Research |
| Dynamic Recycling, Inc. | Manufacturing | $3,549,700 | $3,591,113 | 312 | $87.1M | Research |
| Empire Screen Printing, INC | Manufacturing | $2,223,200 | $2,252,410 | 250 | $248.0M | Research |
| T.i.c., Inc. | Finance and Insurance | $1,647,500 | $1,656,470 | 132 | $67.8M | Research |
| Metallics, INC | Wholesale Trade | $1,282,500 | $1,292,297 | 200 | $61.3M | Research |
| Schneider Heating & Air-Conditioning, Inc. | Construction | $1,204,792 | $1,211,613 | 99 | $31.3M | Research |
| Venture Machine and Tool Inc. | Manufacturing | $1,166,440 | $1,172,467 | 73 | $19.1M | Research |
| Venture Machine and Tool INC | Manufacturing | $1,166,440 | $1,173,115 | 74 | $19.4M | Research |
| Interstate Roofing & Waterproofing, Inc. | Construction | $991,050 | $997,602 | 80 | $26.4M | Research |
| Midwest Farms LLC | Agriculture, Forestry, Fishing and Hunting | $900,713 | $905,228 | 109 | $17.8M | Research |
| Midwest Farms, LLC | Agriculture, Forestry, Fishing and Hunting | $776,325 | $781,089 | 105 | $17.2M | Research |
| Brenengen Chevrolet of La Crosse and Onalaska LLC | Retail Trade | $773,400 | $778,040 | 60 | $15.1M | Research |
| La Crosse Sign Co INC | Manufacturing | $738,400 | $746,694 | 71 | $19.8M | Research |
| Innovia Consulting, Inc. | Wholesale Trade | $736,400 | $740,634 | 51 | $15.6M | Research |
| Dahl Automotive Onalaska, Inc. | Retail Trade | $704,500 | $710,001 | 68 | $17.1M | Research |
| J & K of La Crosse INC | Administrative and Support and Waste Management | $664,958 | $671,312 | 248 | $53.7M | Research |
| Modern Crane Service INC | Construction | $638,400 | $646,043 | 21 | $7.2M | Research |
| Modern Crane Service INC | Construction | $630,540 | $633,390 | 21 | $7.2M | Research |
| Allergy Associates Holdings, Ltd. | Health Care and Social Assistance | $612,000 | $615,236 | 38 | $8.8M | Research |
| Allergy Associates Holdings Ltd. | Health Care and Social Assistance | $507,715 | $512,556 | 36 | $11.3M | Research |
| Ironcore INC | Professional, Scientific, and Technical Services | $457,049 | $460,705 | 31 | $6.4M | Research |
| Hilltopper Refuse & Recycling Service INC | Administrative and Support and Waste Management | $445,400 | $448,431 | 45 | $8.5M | Research |
| Springbrook Community Assisted Living Inc. | Health Care and Social Assistance | $437,100 | $440,330 | 90 | $15.3M | Research |
| Dbs Group, LLC | Construction | $422,200 | $425,894 | 22 | $25.1M | Research |
| Ironcore, INC | Professional, Scientific, and Technical Services | $400,200 | $402,459 | 31 | $6.4M | Research |