| Unified Science LLC | Manufacturing | $681,987 | $684,829 | 50 | $13.1M |  Research |
| Thermal Plastic Design INC | Manufacturing | $545,400 | $548,597 | 56 | $55.5M | Research |
| Thermal Plastic Design INC | Manufacturing | $490,500 | $495,841 | 50 | $49.6M | Research |
| Johnson Motors of St. Croix Falls, Inc. | Retail Trade | $415,700 | $420,256 | 33 | $8.3M | Research |
| Straus Knitting Mills, INC | Manufacturing | $364,000 | $366,184 | 33 | $11.6M | Research |
| Straus Knitting Mills INC | Manufacturing | $327,704 | $329,508 | 30 | $10.6M | Research |
| White Bear Machine, Inc. | Manufacturing | $268,825 | $270,326 | 35 | $8.6M | Research |
| White Bear Machine Inc. | Manufacturing | $268,825 | $270,065 | 35 | $8.6M | Research |
| Auto Dealer Supply LLC | Wholesale Trade | $250,500 | $254,717 | 32 | $9.8M | Research |
| Indianhead Glass INC | Wholesale Trade | $245,500 | $248,460 | 31 | $9.5M | Research |
| Revline, Inc. | Transportation and Warehousing | $241,113 | $247,785 | 18 | <$5M | Research |
| Revline, Inc. | Transportation and Warehousing | $241,000 | $243,833 | 18 | <$5M | Research |
| Dancing Dragonfly, LLC | Accommodation and Food Services | $220,300 | $221,773 | 40 | <$5M | Research |
| Nelson Industries Inc. | Manufacturing | $203,211 | $205,937 | 24 | $6.3M | Research |
| Nelson Industries, Inc. | Manufacturing | $199,700 | $201,425 | 24 | $6.3M | Research |
| St Croix Family Dentistry Sc | Health Care and Social Assistance | $179,000 | $180,591 | 18 | <$5M | Research |
| St. Croix Family Dentistry S.c | Health Care and Social Assistance | $179,000 | $179,928 | 16 | <$5M | Research |
| U.s. Spring Specialties Inc. | Manufacturing | $178,870 | $179,943 | 20 | <$5M | Research |
| U.s. Spring Specialties Inc. | Manufacturing | $165,900 | $166,854 | 18 | <$5M | Research |
| Industrial Tool & Plastics INC | Manufacturing | $135,500 | $136,456 | 14 | $13.9M | Research |
| Dalles Restaurant & Lounge, LLC | Accommodation and Food Services | $130,400 | $131,472 | 41 | $5.0M | Research |
| Douglas Fisk | Transportation and Warehousing | $128,433 | $23,490 | 7 | <$5M | Research |
| Mirachael Management, Inc. | Accommodation and Food Services | $125,300 | $126,261 | 16 | $5.0M | Research |
| Greatland Transportation Systems, INC | Transportation and Warehousing | $119,600 | $120,238 | 12 | <$5M | Research |
| St. Croix River Association | Other Services (except Public Administration) | $115,800 | $116,612 | 9 | N/A | Research |