| Running INC | Transportation and Warehousing | $1,371,275 | $1,387,311 | 386 | $374.4M |  Research |
| Vernon Area Rehabilitation Center, Inc. | Educational Services | $1,271,500 | $1,279,023 | 479 | N/A | Research |
| Bethel Home and Services, Inc. | Health Care and Social Assistance | $1,004,190 | $938,499 | 314 | N/A | Research |
| Sleepy Hollow Chevrolet Buick Gmc INC | Retail Trade | $689,100 | $698,039 | 77 | $19.4M | Research |
| Nelson Mill and Agri-Center, Inc. | Retail Trade | $631,965 | $636,898 | 69 | $14.2M | Research |
| Viroqua Food Cooperative | Retail Trade | $425,400 | $427,875 | 70 | $17.5M | Research |
| Brown Cab Service INC | Transportation and Warehousing | $358,755 | $362,891 | 114 | $110.6M | Research |
| Kickapoo Coffee, INC | Accommodation and Food Services | $320,100 | $322,108 | 41 | $7.3M | Research |
| Moser Trucking INC | Transportation and Warehousing | $247,600 | $250,227 | 14 | <$5M | Research |
| Harmony Valley Farm, LLC | Agriculture, Forestry, Fishing and Hunting | $245,000 | $246,497 | 51 | $5.2M | Research |
| Sleepy Hollow Chrysler Dodge Jeep Inc. | Retail Trade | $241,000 | $244,126 | 39 | $9.8M | Research |
| Cousins Concrete & Construction INC | Construction | $217,100 | $218,517 | 24 | $5.6M | Research |
| Sleepy Hollow Ford Inc. | Retail Trade | $198,100 | $200,670 | 33 | $8.3M | Research |
| Campbell Family Homes LLC | Health Care and Social Assistance | $197,300 | $199,964 | 38 | $6.5M | Research |
| Kickapoo Coffee, INC | Accommodation and Food Services | $188,972 | $190,152 | 15 | <$5M | Research |
| Natures International Certification Services | Agriculture, Forestry, Fishing and Hunting | $187,562 | $189,391 | 22 | <$5M | Research |
| Eitsert Family Care's INC | Health Care and Social Assistance | $171,989 | $174,033 | 28 | <$5M | Research |
| Wiscochef Enterprises LLC | Accommodation and Food Services | $164,107 | $164,868 | 33 | $5.9M | Research |
| Southwest Sanitation LLC | Administrative and Support and Waste Management | $156,900 | $158,085 | 16 | <$5M | Research |
| Vernon Square Cinema, LLC | Accommodation and Food Services | $152,190 | $153,657 | 56 | $9.9M | Research |
| Viroqua Cbrf, LLC | Health Care and Social Assistance | $149,972 | $151,076 | 23 | <$5M | Research |
| Nuzum Building Supply | Retail Trade | $127,101 | $128,072 | 13 | <$5M | Research |
| C&c Landscaping, Inc. | Administrative and Support and Waste Management | $125,000 | $126,500 | 13 | <$5M | Research |
| The Cornucopia Institute INC | Other Services (except Public Administration) | $120,900 | $121,659 | 9 | N/A | Research |
| Nuzum Building Supply, LLC | Retail Trade | $119,543 | $120,628 | 18 | <$5M | Research |