| Region V Board of Cooperative Educational Services | Educational Services | $1,080,700 | $1,090,576 | 86 | N/A |  Research |
| Crystal Springs Ranch Inc. | Construction | $1,074,800 | $1,087,071 | 226 | $70.1M | Research |
| Dembergh Construction Wyoming, Inc. | Construction | $926,200 | $936,182 | 35 | $39.9M | Research |
| Teton Pines LLC | Arts, Entertainment, and Recreation | $687,902 | $696,119 | 110 | $15.4M | Research |
| Teton Gravity Research, LLC | Information | $439,177 | $441,983 | 31 | $5.2M | Research |
| Yellow Iron Excavating, LLC | Construction | $421,900 | $425,099 | 27 | $10.1M | Research |
| Thl Ii, Inc. | Administrative and Support and Waste Management | $344,565 | $347,886 | 72 | $15.6M | Research |
| Rendezvous Rentals LLC | Real Estate and Rental and Leasing | $331,900 | $335,910 | 40 | $6.0M | Research |
| W. J. Grosvenor & Company Inc. | Retail Trade | $326,105 | $328,080 | 21 | <$5M | Research |
| Scarfish LLC | Accommodation and Food Services | $312,987 | $315,161 | 23 | <$5M | Research |
| Aspen Travel INC | Administrative and Support and Waste Management | $306,200 | $307,867 | 24 | $7.6M | Research |
| Bressler Insulation INC | Construction | $302,250 | $304,237 | 20 | $5.1M | Research |
| Hole Food & Entertainment Inc. | Accommodation and Food Services | $278,100 | $279,591 | 46 | $8.6M | Research |
| Shopco Enterprises LLC | Retail Trade | $251,600 | $253,843 | 30 | $7.5M | Research |
| New West Knifeworks, LLC | Retail Trade | $241,300 | $273,027 | 24 | <$5M | Research |
| Aspen Travel Inc. | Administrative and Support and Waste Management | $203,452 | $204,503 | 11 | <$5M | Research |
| Project One Nutrition INC | Retail Trade | $172,627 | $174,862 | 9 | <$5M | Research |
| Bar J Chuckwagon, Inc. | Accommodation and Food Services | $172,200 | $173,511 | 9 | <$5M | Research |
| Granite Ridge Partners | Administrative and Support and Waste Management | $164,862 | $166,222 | 9 | <$5M | Research |
| Nora's Fish Creek Inn Inc. | Accommodation and Food Services | $158,395 | $159,794 | 17 | <$5M | Research |
| Synagile Corporation | Professional, Scientific, and Technical Services | $149,250 | $150,233 | 9 | <$5M | Research |
| Mackay Investments LLC and Moose Wilson Road Campground Inc. | Accommodation and Food Services | $148,200 | $150,056 | 16 | $5.0M | Research |
| Granite Ridge Partners | Administrative and Support and Waste Management | $145,762 | $147,024 | 9 | <$5M | Research |
| Bar J Chuckwagon Inc. | Arts, Entertainment, and Recreation | $142,949 | $144,132 | 9 | <$5M | Research |
| Teton Raptor Center | Professional, Scientific, and Technical Services | $138,135 | $138,714 | 15 | N/A | Research |