| Ology Bioservices, Inc. | Manufacturing | $2,238,732 | $2,183,935 | 217 | $215.2M |  Research |
| Community Markets Inc. | Retail Trade | $1,880,000 | $1,899,792 | 482 | $120.4M | Research |
| The Intermed Group, Inc. | Other Services (except Public Administration) | $1,662,808 | $1,678,388 | 125 | $24.5M | Research |
| Contemporary Management Concepts Lllp | Real Estate and Rental and Leasing | $1,261,400 | $1,267,897 | 144 | $21.6M | Research |
| Causseaux, Hewett & Walpole, Inc. | Professional, Scientific, and Technical Services | $1,097,800 | $1,104,597 | 71 | $8.8M | Research |
| The National Center for Construction Education and Research, | Other Services (except Public Administration) | $1,035,880 | $1,046,009 | 78 | N/A | Research |
| North Central Florida Neurodiagnostic | Health Care and Social Assistance | $962,527 | $975,576 | 102 | $23.6M | Research |
| Reid & Miller, Inc. | Manufacturing | $913,617 | $919,860 | 62 | $16.2M | Research |
| Fracture INC | Real Estate and Rental and Leasing | $872,667 | $845,530 | 79 | $11.8M | Research |
| North Central Florida Neurodiagnostic Services Limited Liabi | Health Care and Social Assistance | $608,275 | $0 | 43 | $10.0M | Research |
| Sante Fe Ford Inc. | Finance and Insurance | $517,100 | $523,420 | 47 | $24.1M | Research |
| Tucker-Davis Technologies, Inc. | Manufacturing | $465,500 | $468,797 | 29 | $7.6M | Research |
| Evergreen Landscape Design, INC | Professional, Scientific, and Technical Services | $422,600 | $425,668 | 53 | $10.7M | Research |
| Dreyer's Cleaning & Restoration Inc. | Administrative and Support and Waste Management | $410,290 | $413,493 | 38 | $8.2M | Research |
| Evergreen Landscape Design Inc. | Administrative and Support and Waste Management | $402,922 | $405,008 | 56 | $12.1M | Research |
| R. E. Arnold Construction, INC | Construction | $400,000 | $379,682 | 54 | $20.2M | Research |
| Alchem Laboratories Corporation | Professional, Scientific, and Technical Services | $345,500 | $347,410 | 22 | <$5M | Research |
| Encell Technology Inc. | Manufacturing | $322,182 | $325,816 | 17 | <$5M | Research |
| Florida Farms INC | Retail Trade | $316,360 | $317,868 | 38 | $7.8M | Research |
| Curtiss Healthcare, Inc. | Manufacturing | $300,300 | $301,978 | 17 | $16.9M | Research |
| Florida Farms Inc. | Agriculture, Forestry, Fishing and Hunting | $294,983 | $265,339 | 36 | <$5M | Research |
| Fdn for Applied Molecular Evolution INC | Professional, Scientific, and Technical Services | $290,315 | $293,910 | 24 | N/A | Research |
| Dreyer's Cleaning & Restoration, Inc. | Administrative and Support and Waste Management | $284,900 | $286,950 | 35 | $7.6M | Research |
| Encell Technology INC | Professional, Scientific, and Technical Services | $264,000 | $267,271 | 20 | <$5M | Research |
| Sgs Contracting Services, Inc. | Construction | $259,448 | $223,139 | 24 | $7.4M | Research |