| Alachua Family Dentistry | Health Care and Social Assistance | $256,470 | $258,678 | 21 | <$5M |  AI Deep Dive |
| Ames/detrick Truck Company | Retail Trade | $250,000 | $251,438 | 26 | $6.8M | AI Deep Dive |
| Lacerta Therapeutics | Wholesale Trade | $249,253 | $0 | 23 | $5.9M | AI Deep Dive |
| Alachua Family Dentistry, P.a. | Health Care and Social Assistance | $246,695 | $249,326 | 20 | <$5M | AI Deep Dive |
| Geoline Surveying | Professional, Scientific, and Technical Services | $228,608 | $230,744 | 19 | <$5M | AI Deep Dive |
| Rbi Electrical Contracting LLC | Wholesale Trade | $224,700 | $226,098 | 23 | $7.0M | AI Deep Dive |
| M&m Metals, LLC | Construction | $210,478 | $212,536 | 21 | $7.2M | AI Deep Dive |
| Firebird Biomolecular Sciences LLC | Professional, Scientific, and Technical Services | $180,200 | $182,623 | 17 | <$5M | AI Deep Dive |
| Dealers Auto Auction, Inc. | Wholesale Trade | $172,897 | $174,517 | 54 | $17.0M | AI Deep Dive |
| Alachua Fl 0716 LLC | Accommodation and Food Services | $163,834 | $164,894 | 21 | $6.6M | AI Deep Dive |
| John C Hipp Construction Equipment Company | Construction | $157,162 | $157,961 | 17 | $5.3M | AI Deep Dive |
| Nextgen Biologics, Inc. | Professional, Scientific, and Technical Services | $155,400 | $156,537 | 15 | <$5M | AI Deep Dive |
| John C Hipp Construction Equip Co | Construction | $149,100 | $150,728 | 18 | $5.6M | AI Deep Dive |
| Conestogas Restaurant | Accommodation and Food Services | $147,000 | $147,636 | 27 | <$5M | AI Deep Dive |
| Rab of Gainesville INC | Accommodation and Food Services | $141,455 | $144,292 | 33 | $5.9M | AI Deep Dive |
| Applied Genetic Technologies Corporation | Manufacturing | $140,895 | $0 | 70 | $69.4M | AI Deep Dive |
| Rab of Gainesville, Inc. | Accommodation and Food Services | $140,300 | $141,672 | 64 | $11.4M | AI Deep Dive |
| Conestogas Restaurant | Accommodation and Food Services | $137,860 | $139,129 | 8 | <$5M | AI Deep Dive |
| Chestnut Hill Nursery, LLC | Agriculture, Forestry, Fishing and Hunting | $137,500 | $138,451 | 21 | <$5M | AI Deep Dive |
| Gator Solutions LLC | Construction | $133,337 | $134,130 | 11 | <$5M | AI Deep Dive |
| New Horizons Professional Lawn Care LLC | Administrative and Support and Waste Management | $129,400 | $130,500 | 18 | <$5M | AI Deep Dive |
| Hillman Supply Company INC | Wholesale Trade | $128,300 | $126,474 | 14 | <$5M | AI Deep Dive |
| Suwannee River Area Health Education Center | Educational Services | $120,343 | $121,850 | 3 | N/A | AI Deep Dive |
| J & K Canine Academy Inc. | Administrative and Support and Waste Management | $118,000 | $100,825 | 13 | <$5M | AI Deep Dive |
| New York Pizza Time, Inc. | Accommodation and Food Services | $117,500 | $89,776 | 17 | <$5M | AI Deep Dive |