| Western Global Airlines LLC | Transportation and Warehousing | $8,197,984 | $8,310,907 | 384 | $97.4M |  Research |
| Naturipe Farms, LLC | Wholesale Trade | $1,817,500 | $1,828,960 | 97 | $25.0M | Research |
| O'donnell Landscapes, Inc. | Professional, Scientific, and Technical Services | $1,252,550 | $1,260,866 | 130 | $26.2M | Research |
| Estero Bay Chevrolet | Retail Trade | $1,140,300 | $1,150,943 | 95 | $23.9M | Research |
| Miromar Development Corporation | Real Estate and Rental and Leasing | $1,024,600 | $1,036,525 | 164 | $24.6M | Research |
| Softrim, LLC | Professional, Scientific, and Technical Services | $1,014,895 | $1,022,648 | 57 | $11.8M | Research |
| Sam Galloway Ford South Ltd | Retail Trade | $953,900 | $0 | 85 | $21.4M | Research |
| Enabling Technologies Corp of Florida | Professional, Scientific, and Technical Services | $849,864 | $856,919 | 56 | $11.6M | Research |
| Pelican Sound Golf and River Club INC | Arts, Entertainment, and Recreation | $800,000 | $807,496 | 80 | $11.2M | Research |
| Spring Run Golf Club Community Assoc INC | Other Services (except Public Administration) | $745,160 | $749,406 | 86 | N/A | Research |
| Professional Contractors Supplies, Inc. | Construction | $731,100 | $735,847 | 37 | $12.6M | Research |
| Ted Todd Insurance INC | Finance and Insurance | $716,368 | $723,237 | 50 | $25.7M | Research |
| Ford's Garage Estero LLC | Accommodation and Food Services | $713,720 | $640,667 | 80 | $14.2M | Research |
| Wildcat Run Country Club Association, INC | Arts, Entertainment, and Recreation | $703,766 | $0 | 91 | $12.7M | Research |
| Ktb Florida Sports Arena LLC | Arts, Entertainment, and Recreation | $697,838 | $706,842 | 232 | $60.9M | Research |
| Ktb Florida Sports Arena LLC | Arts, Entertainment, and Recreation | $697,837 | $706,765 | 232 | $60.9M | Research |
| Grandezza Club 11481 Partners LLC | Arts, Entertainment, and Recreation | $606,889 | $615,166 | 72 | $10.1M | Research |
| Greenway Landscape Supply Inc. | Administrative and Support and Waste Management | $601,800 | $608,303 | 80 | $17.3M | Research |
| 3g Home Exteriors | Retail Trade | $530,188 | $485,573 | 56 | $10.2M | Research |
| Fords Garage Estero LLC | Accommodation and Food Services | $509,800 | $521,826 | 87 | $15.4M | Research |
| The Roofing Brothers of Naples Corp | Construction | $488,098 | $481,766 | 43 | $14.2M | Research |
| Hanania-Richardson Motorcars LLC | Retail Trade | $481,022 | $485,252 | 49 | $12.3M | Research |
| Hanania-Richardson Motorcars LLC | Retail Trade | $481,022 | $485,239 | 29 | $7.3M | Research |
| Miromar Lakes Golf Club LLC | Arts, Entertainment, and Recreation | $412,076 | $416,895 | 57 | $8.0M | Research |
| Elite Supply Chain Services LLC | Transportation and Warehousing | $399,600 | $403,485 | 99 | $19.9M | Research |