| West Coast Eye Institute PA | Health Care and Social Assistance | $561,900 | $568,596 | 51 | $16.0M |  AI Deep Dive |
| Altavian, Inc. | Manufacturing | $555,100 | $587,294 | 43 | $11.3M | AI Deep Dive |
| Scruggs Carmichael & Wershow PA | Professional, Scientific, and Technical Services | $553,500 | $557,190 | 46 | $11.2M | AI Deep Dive |
| Parrish Mccall Constructors, Inc. | Construction | $536,100 | $541,625 | 41 | $46.7M | AI Deep Dive |
| Steven a Bagen & Associates PA | Professional, Scientific, and Technical Services | $523,310 | $527,267 | 38 | $9.2M | AI Deep Dive |
| Trimark Properties, LLC | Wholesale Trade | $522,407 | $525,183 | 54 | $13.9M | AI Deep Dive |
| B & M Equipment Rental & Sales | Real Estate and Rental and Leasing | $508,200 | $507,213 | 44 | $19.9M | AI Deep Dive |
| S Clark Butler Properties Corp | Construction | $502,030 | $509,798 | 42 | $12.8M | AI Deep Dive |
| Aic Administrative Service, LLC | Professional, Scientific, and Technical Services | $500,500 | $504,792 | 42 | $13.4M | AI Deep Dive |
| Gainesville Dermatology and Skin Surgery PA | Health Care and Social Assistance | $493,900 | $497,134 | 62 | $19.5M | AI Deep Dive |
| Vintage Electric, Inc. | Construction | $490,300 | $494,424 | 48 | $15.8M | AI Deep Dive |
| Regenerative Biologics Inc. | Health Care and Social Assistance | $487,500 | $490,750 | 61 | $14.1M | AI Deep Dive |
| Holbrook Travel INC | Administrative and Support and Waste Management | $486,400 | $482,647 | 40 | $12.7M | AI Deep Dive |
| Gainesville Hotels 4 Resorts LLC | Accommodation and Food Services | $483,200 | $487,831 | 40 | $12.5M | AI Deep Dive |
| Akira Wood, Inc. | Manufacturing | $480,500 | $483,944 | 37 | $9.7M | AI Deep Dive |
| Akira Wood Inc. | Construction | $480,078 | $483,158 | 36 | $41.0M | AI Deep Dive |
| Momma G's Inc. | Accommodation and Food Services | $475,749 | $477,969 | 51 | $9.5M | AI Deep Dive |
| Carbonxt INC | Manufacturing | $474,440 | $476,746 | 38 | $10.6M | AI Deep Dive |
| Emmer Management Corp | Professional, Scientific, and Technical Services | $463,977 | $472,651 | 31 | <$5M | AI Deep Dive |
| Carbonxt INC | Public Administration | $461,700 | $466,471 | 46 | N/A | AI Deep Dive |
| Surgical Group of Gainesville, P.a. | Health Care and Social Assistance | $459,500 | $466,405 | 30 | $9.4M | AI Deep Dive |
| Gettel Gainesville INC | Retail Trade | $459,361 | $464,655 | 43 | $10.8M | AI Deep Dive |
| Florida High School Athletic Association, Inc. | Educational Services | $452,300 | $455,529 | 25 | N/A | AI Deep Dive |
| Complete Network Solutions INC | Wholesale Trade | $450,000 | $454,138 | 47 | $14.4M | AI Deep Dive |
| W G Johnson & Son INC | Construction | $446,100 | $447,246 | 33 | $12.4M | AI Deep Dive |