| Stein Mart, Inc. | Retail Trade | $10,000,000 | $10,142,740 | 500 | $151.3M |  Research |
| Nextran Corporation | Wholesale Trade | $10,000,000 | $10,127,123 | 500 | $153.2M | Research |
| Dream Finders Holdings LLC | Construction | $8,760,500 | $7,302,871 | 458 | $139.1M | Research |
| Kls-Martin, LP | Manufacturing | $6,507,272 | $6,584,275 | 336 | $93.8M | Research |
| Enhanced Recovery Company LLC | Administrative and Support and Waste Management | $6,500,000 | $6,635,877 | 500 | $94.6M | Research |
| The Ware Group LLC | Wholesale Trade | $5,891,000 | $5,691,024 | 391 | $119.8M | Research |
| Physicians Group Services PA | Health Care and Social Assistance | $5,414,300 | $5,475,415 | 471 | $147.8M | Research |
| Vesta Property Services, INC | Real Estate and Rental and Leasing | $5,346,949 | $5,409,794 | 500 | $75.0M | Research |
| Ameri-Force Craft Services, Inc. | Manufacturing | $5,154,225 | $5,219,041 | 500 | $130.9M | Research |
| Cancer Specialists LLC | Health Care and Social Assistance | $4,309,600 | $4,364,149 | 326 | $102.3M | Research |
| Pace Center for Girls INC | Educational Services | $4,000,000 | $4,012,667 | 500 | N/A | Research |
| The Bolles School | Educational Services | $3,904,997 | $0 | 332 | N/A | Research |
| The Young Men's Christian Association of Florida's First Coa | Other Services (except Public Administration) | $3,892,022 | $3,187,586 | 500 | $95.5M | Research |
| Tax Defense Network, LLC | Management of Companies and Enterprises | $3,875,600 | $3,915,648 | 219 | $36.0M | Research |
| North Florida Surgeons, P.a. | Health Care and Social Assistance | $3,861,672 | $3,920,348 | 244 | $57.1M | Research |
| Centurion Auto Logistics | Transportation and Warehousing | $3,623,243 | $3,919,599 | 225 | $51.3M | Research |
| Morgan & Morgan Jacksonville Management, Inc. | Administrative and Support and Waste Management | $3,497,565 | $3,539,341 | 334 | $63.2M | Research |
| Interactive Resources LLC | Administrative and Support and Waste Management | $3,490,300 | $3,021,510 | 291 | $45.8M | Research |
| Mcsw Jac Hospitality LLC | Accommodation and Food Services | $3,490,150 | $3,551,716 | 339 | $106.0M | Research |
| Tom Nehl Truck Company | Retail Trade | $3,480,700 | $3,522,565 | 231 | $58.2M | Research |
| Capital Staffing Solutions INC | Administrative and Support and Waste Management | $3,460,708 | $3,499,160 | 349 | $54.9M | Research |
| Farah and Farah, PA | Professional, Scientific, and Technical Services | $3,455,944 | $3,495,522 | 298 | $72.4M | Research |
| Acme Barricades, Lc | Real Estate and Rental and Leasing | $3,413,500 | $3,454,367 | 319 | $144.1M | Research |
| Tom Bush Volkswagen, INC | Retail Trade | $3,294,108 | $3,336,656 | 282 | $71.1M | Research |
| England, Thims & Miller, Inc. | Professional, Scientific, and Technical Services | $3,198,700 | $0 | 182 | $22.4M | Research |