| Arlington Motor Company INC | Retail Trade | $3,179,242 | $3,216,421 | 241 | $60.7M | AI Deep Dive |
| Beaver Street Fisheries INC | Wholesale Trade | $3,155,099 | $3,198,060 | 173 | $44.5M | AI Deep Dive |
| Alluvion Staffing, Inc. | Administrative and Support and Waste Management | $3,148,655 | $3,185,127 | 302 | $47.5M | AI Deep Dive |
| Diocese of Saint Augustine INC | Other Services (except Public Administration) | $3,139,500 | $1,267,043 | 115 | N/A | AI Deep Dive |
| Shippers Automotive Group LLC | Professional, Scientific, and Technical Services | $3,128,700 | $3,162,559 | 268 | $27.7M | AI Deep Dive |
| Heart Utilities of Jacksonville, Inc. | Public Administration | $3,118,000 | $3,157,408 | 125 | N/A | AI Deep Dive |
| Intuition LLC | Public Administration | $3,091,000 | $2,246,889 | 241 | N/A | AI Deep Dive |
| Nimnicht Chevrolet Company | Retail Trade | $3,084,500 | $3,161,184 | 215 | $54.2M | AI Deep Dive |
| Owens Lawn Care | Real Estate and Rental and Leasing | $3,000,000 | $3,034,521 | 346 | $50.2M | AI Deep Dive |
| Trailer Bridge INC | Transportation and Warehousing | $2,969,823 | $3,002,288 | 220 | $44.3M | AI Deep Dive |
| Load King Manufacturing Co. | Manufacturing | $2,895,815 | $2,928,956 | 179 | $46.9M | AI Deep Dive |
| River City Education Services, Inc. | Educational Services | $2,878,600 | $2,917,301 | 330 | N/A | AI Deep Dive |
| The Yerba Mate Co LLC | Wholesale Trade | $2,843,200 | $2,860,496 | 300 | $77.2M | AI Deep Dive |
| Brightway Insurance, Inc. | Finance and Insurance | $2,810,420 | $2,843,165 | 254 | $130.5M | AI Deep Dive |
| Manning Building Supplies INC | Wholesale Trade | $2,761,285 | $2,459,462 | 288 | $88.2M | AI Deep Dive |
| Milton J Wood Company | Construction | $2,750,000 | $2,808,438 | 200 | $63.3M | AI Deep Dive |
| Complete Health Partners, Inc. | Health Care and Social Assistance | $2,691,105 | $2,720,818 | 212 | $49.1M | AI Deep Dive |
| Episcopal School of Jacksonville INC | Educational Services | $2,653,500 | $2,683,426 | 180 | N/A | AI Deep Dive |
| Southeast Mechanical Systems, Inc. | Construction | $2,642,800 | $2,673,192 | 194 | $61.4M | AI Deep Dive |
| Florida Physician Specialists LLC | Health Care and Social Assistance | $2,641,200 | $2,016,098 | 153 | $48.0M | AI Deep Dive |
| River Garden Hebrew Home for the Aged | Health Care and Social Assistance | $2,638,034 | $1,987,804 | 315 | N/A | AI Deep Dive |
| Family Care Partners of Northeast Fl, LLC | Health Care and Social Assistance | $2,573,200 | $2,601,188 | 233 | $73.1M | AI Deep Dive |
| Eisman & Russo INC | Professional, Scientific, and Technical Services | $2,500,000 | $0 | 152 | $18.7M | AI Deep Dive |
| Tech Packaging INC | Manufacturing | $2,500,000 | $1,817,085 | 250 | $88.2M | AI Deep Dive |
| F M X, INC | Transportation and Warehousing | $2,500,000 | $254,976 | 22 | <$5M | AI Deep Dive |