| Preservation Home Specialists, Inc. | Professional, Scientific, and Technical Services | $10,000,000 | $158,014 | 18 | <$5M |  Research |
| Optimum Healthcare It LLC | Professional, Scientific, and Technical Services | $7,274,497 | $7,333,501 | 364 | $75.3M | Research |
| Jax Refrigeration, LLC | Wholesale Trade | $3,902,768 | $3,948,639 | 181 | $55.4M | Research |
| Hueman People Solutions LLC | Professional, Scientific, and Technical Services | $2,074,732 | $2,102,510 | 157 | $16.2M | Research |
| Jax Refrigeration, LLC | Wholesale Trade | $2,000,000 | $2,016,822 | 145 | $44.4M | Research |
| The Church of Eleven22, Inc. | Other Services (except Public Administration) | $1,730,965 | $1,749,669 | 211 | N/A | Research |
| The Favergray Company | Construction | $1,514,000 | $1,527,205 | 82 | $24.9M | Research |
| Richard L. Oreair & Company | Construction | $1,146,878 | $1,100,822 | 56 | $17.7M | Research |
| Earth Systems Inc. | Professional, Scientific, and Technical Services | $1,014,855 | $1,025,201 | 77 | $15.5M | Research |
| Adventure Holdings, LLC | Arts, Entertainment, and Recreation | $962,203 | $0 | 347 | $48.6M | Research |
| Cantina Louie Corporate, LLC | Accommodation and Food Services | $944,340 | $957,902 | 163 | $28.9M | Research |
| First Coast Beverages INC | Accommodation and Food Services | $910,494 | $920,547 | 97 | $11.9M | Research |
| Salus Medical Consultants, LLC | Health Care and Social Assistance | $903,784 | $94,184 | 11 | <$5M | Research |
| 86 the Q Inc. | Accommodation and Food Services | $807,738 | $815,085 | 125 | $22.2M | Research |
| Jd-Asg Mgmt Co., Inc. | Accommodation and Food Services | $719,700 | $724,518 | 277 | $49.2M | Research |
| North Beach Fish Camp Inc. | Accommodation and Food Services | $675,900 | $682,418 | 71 | $12.6M | Research |
| A Care Connection, LLC | Health Care and Social Assistance | $636,974 | $37,668 | 57 | $9.8M | Research |
| M3 Govcon, LLC | Administrative and Support and Waste Management | $625,200 | $631,858 | 42 | $7.9M | Research |
| Atlantic Eye Institute, P.a. | Health Care and Social Assistance | $622,812 | $628,072 | 64 | $20.1M | Research |
| Sawgrass Asset Management LLC | Finance and Insurance | $617,099 | $621,427 | 19 | $9.8M | Research |
| Sawgrass Asset Management, LLC | Finance and Insurance | $617,000 | $623,542 | 23 | $11.8M | Research |
| Trade Show Technologies Inc. | Transportation and Warehousing | $602,532 | $608,507 | 24 | $9.3M | Research |
| Trade Show Technologies, Inc. | Professional, Scientific, and Technical Services | $594,260 | $601,044 | 40 | $5.6M | Research |
| 86 the Q, Inc. | Accommodation and Food Services | $592,800 | $599,426 | 120 | $21.3M | Research |
| Diocese of St. Augustine, Inc. | Educational Services | $583,365 | $589,053 | 99 | N/A | Research |