| Florida Baptist Convention INC | Other Services (except Public Administration) | $650,000 | $655,200 | 50 | $9.6M | AI Deep Dive |
| Westside Electric INC | Construction | $649,147 | $656,706 | 56 | $18.4M | AI Deep Dive |
| Concrete Conservation LLC | Manufacturing | $644,800 | $649,287 | 39 | $9.5M | AI Deep Dive |
| Southside-Jax Mellow LLC | Accommodation and Food Services | $644,707 | $653,009 | 63 | $11.2M | AI Deep Dive |
| Joel a David & Associates PA | Health Care and Social Assistance | $643,253 | $651,937 | 51 | $11.8M | AI Deep Dive |
| United Brothers Development Corporation | Construction | $642,629 | $648,538 | 63 | $23.6M | AI Deep Dive |
| Tc Motorcycles LLC | Retail Trade | $640,940 | $648,373 | 67 | $17.5M | AI Deep Dive |
| Tntsi Inc. | Administrative and Support and Waste Management | $638,900 | $644,171 | 36 | $5.3M | AI Deep Dive |
| Kls Martin Manufacturing, LLC | Manufacturing | $638,140 | $645,106 | 56 | $15.6M | AI Deep Dive |
| Jacksonville Ss Automotive Management, LLC | Retail Trade | $637,900 | $598,229 | 59 | $14.9M | AI Deep Dive |
| Dns Contracting, LLC | Construction | $637,500 | $642,706 | 55 | $17.0M | AI Deep Dive |
| Elo Restoration INC | Construction | $636,200 | $641,678 | 60 | $19.8M | AI Deep Dive |
| Dynamic Dental Solutions Inc. | Manufacturing | $636,000 | $639,374 | 51 | $14.2M | AI Deep Dive |
| Orion Solutions LLC | Professional, Scientific, and Technical Services | $631,800 | $635,799 | 56 | $6.9M | AI Deep Dive |
| Physicians Trust INC | Finance and Insurance | $631,141 | $636,944 | 35 | $18.0M | AI Deep Dive |
| Ultra Tech International Inc. | Wholesale Trade | $630,800 | $633,721 | 39 | $10.0M | AI Deep Dive |
| Military Construction Corporation | Construction | $630,000 | $633,500 | 48 | $54.7M | AI Deep Dive |
| North Florida School of Special Education | Public Administration | $629,945 | $633,760 | 101 | N/A | AI Deep Dive |
| Etm Surveying & Mapping, Inc. | Professional, Scientific, and Technical Services | $629,200 | $0 | 57 | $11.5M | AI Deep Dive |
| Hiday & Ricke, PA | Professional, Scientific, and Technical Services | $628,255 | $632,408 | 56 | $13.6M | AI Deep Dive |
| International Baler Corporation | Manufacturing | $626,466 | $629,899 | 47 | $12.3M | AI Deep Dive |
| Cowford Chophouse | Accommodation and Food Services | $626,103 | $631,849 | 95 | $16.9M | AI Deep Dive |
| Flat Glass Distributors LLC | Manufacturing | $625,900 | $633,254 | 69 | $68.4M | AI Deep Dive |
| James Michael Howard INC | Professional, Scientific, and Technical Services | $625,227 | $628,002 | 52 | $7.1M | AI Deep Dive |
| B.c. Industrial Supply, Inc. | Wholesale Trade | $625,020 | $629,378 | 42 | $12.9M | AI Deep Dive |