| Sea Breeze Service INC | Wholesale Trade | $1,181,950 | $1,195,977 | 110 | $28.3M | AI Deep Dive |
| Auto Carrier Express, Inc. | Transportation and Warehousing | $1,181,900 | $1,193,489 | 69 | $15.7M | AI Deep Dive |
| The Arc Jacksonville Inc. | Health Care and Social Assistance | $1,175,940 | $1,182,287 | 203 | $34.5M | AI Deep Dive |
| Nfc Logistics, Llc. | Transportation and Warehousing | $1,175,700 | $1,189,357 | 185 | $43.1M | AI Deep Dive |
| Corporate Traffic, Inc. | Transportation and Warehousing | $1,173,848 | $1,185,361 | 75 | $15.1M | AI Deep Dive |
| Live Oak Contracting, LLC | Mining, Quarrying, and Oil and Gas Extraction | $1,173,232 | $871,710 | 61 | <$5M | AI Deep Dive |
| Finxact INC | Professional, Scientific, and Technical Services | $1,168,900 | $1,181,044 | 74 | $15.3M | AI Deep Dive |
| First Coast Heart & Vascular Center P.a. | Health Care and Social Assistance | $1,168,500 | $831,660 | 87 | $27.3M | AI Deep Dive |
| Coastal Industries INC of Jack | Wholesale Trade | $1,158,086 | $1,167,254 | 172 | $52.7M | AI Deep Dive |
| Omni Jacksonville Corporation | Accommodation and Food Services | $1,153,700 | $1,167,192 | 157 | $49.1M | AI Deep Dive |
| Communities in Schools of Jacksonville I | Health Care and Social Assistance | $1,152,929 | $1,026,672 | 338 | N/A | AI Deep Dive |
| The Medical Imaging Partnership- Jax1 LLC | Health Care and Social Assistance | $1,142,394 | $1,149,116 | 82 | $15.0M | AI Deep Dive |
| Enterprise Integration Inc. | Professional, Scientific, and Technical Services | $1,134,032 | $1,145,309 | 85 | $17.6M | AI Deep Dive |
| Brooks Building Solutions, Inc. | Construction | $1,125,800 | $1,124,790 | 72 | $22.8M | AI Deep Dive |
| Certified Air Contractors INC | Construction | $1,120,200 | $0 | 67 | $22.0M | AI Deep Dive |
| Smith Hulsey & Busey | Professional, Scientific, and Technical Services | $1,111,232 | $1,117,899 | 60 | $14.6M | AI Deep Dive |
| Munson and Bryan Electric Company, Inc. | Construction | $1,110,636 | $1,121,681 | 80 | $26.3M | AI Deep Dive |
| Kenworth of Jacksonville Inc. | Wholesale Trade | $1,108,877 | $1,123,508 | 84 | $25.7M | AI Deep Dive |
| Williams & Rowe Company Inc. | Construction | $1,108,000 | $1,116,248 | 99 | $112.8M | AI Deep Dive |
| Celebration Church of Jacksonville Inc. | Other Services (except Public Administration) | $1,106,400 | $0 | 259 | N/A | AI Deep Dive |
| Florida Roads Contracting LLC | Construction | $1,103,500 | $1,109,018 | 119 | $36.9M | AI Deep Dive |
| Florida Roads Contracting LLC | Construction | $1,103,500 | $1,111,010 | 111 | $34.4M | AI Deep Dive |
| True House, Inc. | Manufacturing | $1,101,736 | $1,115,630 | 186 | $184.5M | AI Deep Dive |
| Smart Pharmacy | Retail Trade | $1,100,585 | $1,112,080 | 53 | $9.2M | AI Deep Dive |
| Dcc Lee Enterprises, INC | Accommodation and Food Services | $1,090,600 | $1,097,592 | 140 | $24.9M | AI Deep Dive |