| U.s. Air Conditioning Heating & Refrigeration Corp | Wholesale Trade | $310,437 | $313,895 | 39 | $11.9M |  Research |
| Jacobs Auto Enterprise, INC | Retail Trade | $309,539 | $311,465 | 40 | $10.1M | Research |
| The Chapel Church, Inc. | Other Services (except Public Administration) | $303,600 | $306,973 | 28 | N/A | Research |
| First Baptist Church of Elfers Florida INC | Other Services (except Public Administration) | $298,100 | $300,046 | 54 | N/A | Research |
| The Red Apple School, Inc. | Educational Services | $296,100 | $297,778 | 39 | N/A | Research |
| The Red Apple School Inc. | Health Care and Social Assistance | $292,343 | $293,772 | 48 | N/A | Research |
| Amy Kelly | Administrative and Support and Waste Management | $291,255 | $0 | 45 | $9.7M | Research |
| Timber Greens Community Association, INC | Arts, Entertainment, and Recreation | $288,600 | $290,356 | 49 | $6.9M | Research |
| Oceanside Restaurant Group LLC | Accommodation and Food Services | $288,015 | $289,799 | 50 | $8.9M | Research |
| T & R Painting Specialists Inc. | Construction | $287,500 | $290,863 | 20 | $5.1M | Research |
| T & R Painting Specialists INC | Construction | $287,500 | $290,295 | 30 | $7.6M | Research |
| Four Murphys LLC | Health Care and Social Assistance | $275,900 | $277,944 | 63 | $10.2M | Research |
| St-Ra, Inc. | Unknown | $275,467 | $277,184 | 78 | N/A | Research |
| C.r.s. Medical Benefits, Inc. | Professional, Scientific, and Technical Services | $273,467 | $275,894 | 34 | $10.8M | Research |
| C.r.s. Medical Benefits Inc. | Administrative and Support and Waste Management | $273,467 | $275,063 | 20 | <$5M | Research |
| Suncoast Urgent Care Centers LLC | Health Care and Social Assistance | $270,800 | $274,320 | 33 | $10.4M | Research |
| Suncoast Urgent Care Centers LLC | Health Care and Social Assistance | $270,800 | $273,478 | 33 | $10.4M | Research |
| Bruce N L | Unknown | $269,518 | $274,901 | 6 | N/A | Research |
| Western Florida Lighting LLC | Wholesale Trade | $262,500 | $265,909 | 18 | $5.5M | Research |
| Rajn Hotels LLC | Accommodation and Food Services | $258,800 | $260,842 | 32 | $10.0M | Research |
| Gulf Coast Dentistry | Health Care and Social Assistance | $254,503 | $256,729 | 25 | $5.8M | Research |
| Ewing and Thomas, Inc. | Health Care and Social Assistance | $252,200 | $256,074 | 24 | $6.5M | Research |
| Fl Sunshine Services of Tampa, LLC | Administrative and Support and Waste Management | $250,000 | $252,528 | 40 | $8.7M | Research |
| Pielak and Horwitz Dentistry | Health Care and Social Assistance | $231,536 | $233,166 | 19 | <$5M | Research |
| First Christian Academy and Learning Center | Educational Services | $228,300 | $229,816 | 40 | N/A | Research |