| New American Physical Therapy INC | Health Care and Social Assistance | $167,123 | $168,906 | 16 | <$5M |  Research |
| Gator Security INC | Administrative and Support and Waste Management | $163,901 | $164,948 | 14 | <$5M | Research |
| Brad Goodbread Trucking, LLC | Transportation and Warehousing | $162,865 | $164,574 | 20 | $5.3M | Research |
| Superior Water Works INC | Utilities | $155,750 | $157,478 | 19 | N/A | Research |
| Superior Water Works INC | Utilities | $155,200 | $156,446 | 20 | N/A | Research |
| Lightsey's Restaurant Inc. | Accommodation and Food Services | $151,246 | $152,153 | 56 | $9.9M | Research |
| El Primo Wireless Phones INC | Information | $148,750 | $150,266 | 21 | <$5M | Research |
| Crewsville Dairy, Inc. | Agriculture, Forestry, Fishing and Hunting | $148,640 | $149,532 | 15 | <$5M | Research |
| Wayne Carr Trucking Inc. | Transportation and Warehousing | $146,100 | $147,369 | 17 | <$5M | Research |
| A Child's World Childcare and Preschool, Inc. | Health Care and Social Assistance | $145,300 | $146,219 | 28 | <$5M | Research |
| Cyber Electric of Central Florida INC | Construction | $144,250 | $145,463 | 17 | $5.6M | Research |
| A C Hild's World Childcare and Preschool Inc. | Health Care and Social Assistance | $144,233 | $144,940 | 28 | <$5M | Research |
| Crewsville Dairy, Inc. | Agriculture, Forestry, Fishing and Hunting | $142,087 | $143,137 | 15 | <$5M | Research |
| Display Systems, Inc. | Information | $142,000 | $143,243 | 14 | <$5M | Research |
| K.a. Driggers and Sons Constructioninc | Construction | $138,165 | $139,959 | 40 | $12.4M | Research |
| Adron Fence Company | Construction | $134,000 | $134,767 | 17 | $5.8M | Research |
| Cyber Electric of Central Florida INC | Construction | $132,175 | $133,240 | 18 | $5.9M | Research |
| B&b Site Development, Inc. | Construction | $132,000 | $133,712 | 19 | $5.9M | Research |
| United Feed Co-Op INC | Wholesale Trade | $131,700 | $133,097 | 10 | <$5M | Research |
| Wemmer Family Orthodontics, P.a. | Health Care and Social Assistance | $131,400 | $132,674 | 62 | $14.4M | Research |
| Gator Feed Co., Inc. | Manufacturing | $130,500 | $131,820 | 16 | $5.6M | Research |
| Family Dentistry of Okeechobee | Health Care and Social Assistance | $129,200 | $130,244 | 15 | <$5M | Research |
| Native-Co Environmental, LLC | Administrative and Support and Waste Management | $128,840 | $32,589 | 9 | <$5M | Research |
| Atland Recycling INC | Wholesale Trade | $128,745 | $129,718 | 19 | $5.8M | Research |
| Tapia Nursery INC | Wholesale Trade | $127,600 | $115,076 | 25 | $6.4M | Research |