| Roy H. Hinman, M.d., P.a. | Health Care and Social Assistance | $4,398,881 | $4,453,011 | 371 | $116.4M |  Research |
| The Stageline Company | Transportation and Warehousing | $4,066,100 | $4,111,774 | 456 | $68.9M | Research |
| Dlp Real Estate Capital INC | Real Estate and Rental and Leasing | $3,960,300 | $3,734,071 | 314 | $57.5M | Research |
| Bazard Ford Company | Retail Trade | $2,930,900 | $2,964,224 | 230 | $58.0M | Research |
| Watson Civil Construction, Inc. | Construction | $2,461,000 | $2,489,780 | 176 | $54.6M | Research |
| Beaver Motors, INC | Retail Trade | $2,008,975 | $2,032,469 | 123 | $31.0M | Research |
| All Reliable Services Inc. | Construction | $2,000,000 | $2,012,611 | 233 | $79.4M | Research |
| Rulon Company | Manufacturing | $1,964,385 | $1,984,298 | 169 | $167.6M | Research |
| Owen Electric Company INC | Professional, Scientific, and Technical Services | $1,875,130 | $1,902,684 | 163 | $22.8M | Research |
| The Arc of the St. Johns, Inc. | Health Care and Social Assistance | $1,861,100 | $1,877,213 | 215 | N/A | Research |
| Vista Hospitality Management INC | Administrative and Support and Waste Management | $1,704,800 | $238,828 | 297 | $56.2M | Research |
| St Johns County Welfare Federation | Health Care and Social Assistance | $1,608,800 | $1,629,252 | 175 | N/A | Research |
| World Golf Foundation INC | Other Services (except Public Administration) | $1,545,207 | $1,556,796 | 90 | N/A | Research |
| A/c Designs of St. Augustine, Inc. | Construction | $1,477,000 | $1,489,018 | 133 | $42.1M | Research |
| Ideal Deals, LLC | Construction | $1,315,067 | $1,324,309 | 128 | $43.6M | Research |
| St Augustine Historical Tours, Inc. | Administrative and Support and Waste Management | $1,145,500 | $1,162,110 | 159 | $50.3M | Research |
| Rekcut Photographic INC | Professional, Scientific, and Technical Services | $1,145,121 | $1,154,250 | 208 | $24.7M | Research |
| Rekcut Photographic INC | Other Services (except Public Administration) | $1,145,100 | $1,158,682 | 208 | $39.7M | Research |
| Big Splash Restaurant Group LLC | Accommodation and Food Services | $1,134,653 | $1,141,274 | 130 | $23.1M | Research |
| Orthopeadic Associates of St. Augustine | Health Care and Social Assistance | $1,111,605 | $1,127,384 | 85 | $16.9M | Research |
| Stone Poney INC | Accommodation and Food Services | $1,091,185 | $1,095,161 | 170 | $30.2M | Research |
| Pizzalley's, LLC | Accommodation and Food Services | $1,052,500 | $1,065,218 | 209 | $37.1M | Research |
| Burkhardt Distributing Company INC | Wholesale Trade | $1,032,717 | $1,043,331 | 94 | $24.2M | Research |
| Riley Gear Corporation | Manufacturing | $910,892 | $925,017 | 66 | $17.3M | Research |
| Cutting Edge Welding & Fabricating INC | Construction | $893,100 | $902,716 | 80 | $18.5M | Research |