| Ponce Nh LLC | Health Care and Social Assistance | $881,737 | $892,076 | 111 | $18.9M |  Research |
| Get in Touch, Inc. | Information | $849,500 | $722,447 | 68 | $11.4M | Research |
| St. Johns County Council On Aging, Inc. | Health Care and Social Assistance | $832,500 | $837,723 | 160 | N/A | Research |
| Big Splash Restaurant Group, LLC | Wholesale Trade | $810,467 | $818,194 | 157 | $40.4M | Research |
| H1 St. Augustine Motors, LLC | Retail Trade | $787,137 | $796,108 | 62 | $15.6M | Research |
| Stone Poney INC | Accommodation and Food Services | $779,418 | $786,892 | 155 | $27.5M | Research |
| D3 Air and Space Operations INC | Professional, Scientific, and Technical Services | $778,397 | $783,110 | 54 | $7.6M | Research |
| Smith Trucking Company INC | Mining, Quarrying, and Oil and Gas Extraction | $775,300 | $781,736 | 61 | <$5M | Research |
| Smith Trucking Company Inc. | Construction | $748,307 | $751,690 | 61 | $18.9M | Research |
| Big Splash Fernandina Beach, LLC | Accommodation and Food Services | $704,945 | $711,820 | 123 | $21.8M | Research |
| Gastroenterology Associates of St. Augustine, PA | Health Care and Social Assistance | $691,600 | $537,285 | 41 | $12.9M | Research |
| Epic Community Services INC | Health Care and Social Assistance | $686,500 | $693,854 | 92 | N/A | Research |
| Honours - World Golf Village LLC | Arts, Entertainment, and Recreation | $654,778 | $661,702 | 45 | $6.3M | Research |
| Atlantic Dodge Chrysler Jeep Inc. | Retail Trade | $641,100 | $647,862 | 10 | <$5M | Research |
| Physicians Exclusive LLC DBA Microbiome | Unknown | $632,500 | $640,782 | 41 | N/A | Research |
| Ddn St. Johns, LLC | Retail Trade | $610,928 | $615,798 | 61 | $15.4M | Research |
| Transcription, Technology & Support | Administrative and Support and Waste Management | $595,000 | $603,115 | 150 | $28.4M | Research |
| Kevon E. Rennie Dmd, P.a. | Health Care and Social Assistance | $591,632 | $595,085 | 44 | $10.2M | Research |
| Conch House Builders Ii, LLC | Accommodation and Food Services | $590,282 | $596,693 | 60 | $18.8M | Research |
| Nte Energy Services Company, LLC | Professional, Scientific, and Technical Services | $580,000 | $586,332 | 18 | <$5M | Research |
| St Augustine Youth Services INC | Health Care and Social Assistance | $576,500 | $581,223 | 74 | N/A | Research |
| Rail Scale INC | Transportation and Warehousing | $575,075 | $578,715 | 38 | <$5M | Research |
| Flagler Family Medicine PA | Health Care and Social Assistance | $561,097 | $566,078 | 48 | $15.1M | Research |
| Safety Shelter of St. Johns County INC | Other Services (except Public Administration) | $555,600 | $559,801 | 65 | N/A | Research |
| Path Solution LLC | Information | $546,800 | $0 | 45 | $7.6M | Research |