| Iowa Tanklines, Inc. | Wholesale Trade | $582,552 | $587,908 | 49 | $12.6M |  AI Deep Dive |
| Iowa Association of Municipal Utilities | Other Services (except Public Administration) | $554,575 | $556,793 | 34 | $6.5M | AI Deep Dive |
| Aspen Aire, Inc. | Construction | $542,600 | $549,364 | 59 | $18.7M | AI Deep Dive |
| Jjc Ankeny LLC | Accommodation and Food Services | $510,958 | $0 | 40 | $7.1M | AI Deep Dive |
| Mlb of Iowa INC | Retail Trade | $489,670 | $492,853 | 47 | $9.7M | AI Deep Dive |
| Main Street Cafe and Bakery INC | Accommodation and Food Services | $481,289 | $485,786 | 71 | $12.6M | AI Deep Dive |
| Rognes Corp | Construction | $460,024 | $463,477 | 30 | $9.3M | AI Deep Dive |
| Premier Plumbing INC | Wholesale Trade | $438,900 | $441,338 | 42 | $12.9M | AI Deep Dive |
| Jt Logistics Solutions, LLC | Transportation and Warehousing | $435,000 | $439,922 | 44 | $11.2M | AI Deep Dive |
| Jt Logistics Solutions LLC | Transportation and Warehousing | $435,000 | $438,945 | 44 | $11.2M | AI Deep Dive |
| Halbrook Excavating, Inc. | Construction | $428,403 | $431,795 | 43 | $16.1M | AI Deep Dive |
| On the Waterfront Seafood Restaurant | Accommodation and Food Services | $424,932 | $428,436 | 57 | $10.1M | AI Deep Dive |
| Tender Years Daycare Inc. | Health Care and Social Assistance | $421,130 | $424,347 | 55 | $8.9M | AI Deep Dive |
| Tender Years Daycare Center, Inc. | Health Care and Social Assistance | $421,130 | $426,160 | 89 | $14.4M | AI Deep Dive |
| Acute Care, Inc. | Health Care and Social Assistance | $414,085 | $417,927 | 28 | <$5M | AI Deep Dive |
| Lasting Impressions Growing and Learning Center | Health Care and Social Assistance | $394,800 | $397,706 | 110 | $17.8M | AI Deep Dive |
| Pratum, Inc. | Professional, Scientific, and Technical Services | $393,900 | $396,209 | 25 | $5.2M | AI Deep Dive |
| Ankeny Hotel Associates LLC | Accommodation and Food Services | $371,896 | $374,779 | 59 | $18.4M | AI Deep Dive |
| Exec 1 Aviation Ii, LLC | Transportation and Warehousing | $361,900 | $364,131 | 30 | <$5M | AI Deep Dive |
| Jcorp, Inc. | Professional, Scientific, and Technical Services | $352,941 | $356,363 | 20 | $6.2M | AI Deep Dive |
| Main Street Cafe and Bakery | Accommodation and Food Services | $346,180 | $349,291 | 34 | $6.0M | AI Deep Dive |
| Ankeny Cold Storage, LLC | Transportation and Warehousing | $337,900 | $339,805 | 56 | $14.2M | AI Deep Dive |
| Ankeny Christian Academy | Educational Services | $336,900 | $338,737 | 44 | N/A | AI Deep Dive |
| Hintz Family Dentistry P.c. | Health Care and Social Assistance | $328,227 | $330,944 | 20 | <$5M | AI Deep Dive |
| Hintz Family Dentistry, PC | Health Care and Social Assistance | $328,092 | $330,760 | 47 | $10.9M | AI Deep Dive |