| St Luke the Evangelist Catholic Church | Other Services (except Public Administration) | $228,504 | $229,912 | 34 | N/A | AI Deep Dive |
| Paducah Gear Machine Co. L.c. | Other Services (except Public Administration) | $228,405 | $230,042 | 14 | <$5M | AI Deep Dive |
| The Other Place Ankeny Inc. | Accommodation and Food Services | $224,784 | $227,450 | 20 | <$5M | AI Deep Dive |
| Thorpe Water Development | Construction | $224,000 | $226,060 | 11 | $12.5M | AI Deep Dive |
| Water Development Co | Construction | $224,000 | $225,257 | 11 | $12.5M | AI Deep Dive |
| Premier Crete Inc. | Construction | $223,703 | $226,075 | 18 | <$5M | AI Deep Dive |
| Ankeny Lil Tots Daycare & Preschool | Health Care and Social Assistance | $223,200 | $226,312 | 40 | $6.5M | AI Deep Dive |
| Metro Eyecare Associates, LLC | Unknown | $220,900 | $222,612 | 21 | N/A | AI Deep Dive |
| Doextra Corporation | Information | $220,870 | $224,404 | 13 | <$5M | AI Deep Dive |
| Greenland Homes INC | Construction | $219,655 | $221,748 | 21 | $6.4M | AI Deep Dive |
| Ankeny Dental Associates, P.c. | Health Care and Social Assistance | $217,400 | $219,338 | 15 | <$5M | AI Deep Dive |
| Shibuya Dinning Group LLC | Accommodation and Food Services | $215,527 | $217,729 | 38 | $6.7M | AI Deep Dive |
| Urban Hospitality | Accommodation and Food Services | $214,435 | $216,268 | 34 | $6.0M | AI Deep Dive |
| Lighting Solutions of Iowa, Inc. | Wholesale Trade | $214,093 | $215,483 | 14 | <$5M | AI Deep Dive |
| Freedom Home Health Care INC | Health Care and Social Assistance | $212,500 | $214,514 | 15 | <$5M | AI Deep Dive |
| Ankeny Dental Associates P.c. | Health Care and Social Assistance | $212,479 | $214,161 | 25 | $5.8M | AI Deep Dive |
| Ankeny First United Methodist Church | Other Services (except Public Administration) | $210,000 | $211,651 | 14 | N/A | AI Deep Dive |
| Anani Salon and Spa INC | Other Services (except Public Administration) | $209,500 | $211,142 | 20 | <$5M | AI Deep Dive |
| Tnt Enterprises INC | Unknown | $206,539 | $208,802 | 34 | N/A | AI Deep Dive |
| Wonderdogs INC | Accommodation and Food Services | $206,314 | $207,360 | 33 | $5.9M | AI Deep Dive |
| 72 Heating & Cooling, Inc. | Construction | $206,000 | $208,031 | 18 | $5.7M | AI Deep Dive |
| Anani Salon and Spa | Other Services (except Public Administration) | $204,265 | $206,615 | 20 | <$5M | AI Deep Dive |
| Briarwood Membership Association, INC | Arts, Entertainment, and Recreation | $203,485 | $205,269 | 21 | <$5M | AI Deep Dive |
| D & D Fitness, L.l.c. | Arts, Entertainment, and Recreation | $203,164 | $205,497 | 23 | <$5M | AI Deep Dive |
| D & D Fitness L.l.c | Arts, Entertainment, and Recreation | $203,164 | $203,793 | 23 | <$5M | AI Deep Dive |