| Image INC | Real Estate and Rental and Leasing | $174,400 | $175,446 | 23 | <$5M | AI Deep Dive |
| C&a Transportation & Logistics INC | Transportation and Warehousing | $174,300 | $175,761 | 12 | <$5M | AI Deep Dive |
| Perennial Gardens INC | Professional, Scientific, and Technical Services | $173,400 | $174,441 | 15 | <$5M | AI Deep Dive |
| II for You, LLC | Health Care and Social Assistance | $172,500 | $174,168 | 12 | <$5M | AI Deep Dive |
| Generativity, Lc | Health Care and Social Assistance | $168,095 | $169,580 | 40 | $6.8M | AI Deep Dive |
| Southeast Creekview Hotel Associates LLC | Accommodation and Food Services | $165,249 | $166,503 | 33 | $10.3M | AI Deep Dive |
| Dapro, L.l.c. | Manufacturing | $163,300 | $164,114 | 14 | <$5M | AI Deep Dive |
| Cruiser Electric LLC | Construction | $160,630 | $161,241 | 14 | <$5M | AI Deep Dive |
| Kp Enterprises Iowa, LLC | Health Care and Social Assistance | $158,670 | $160,208 | 35 | $8.2M | AI Deep Dive |
| The Other Place Ankeny INC | Accommodation and Food Services | $157,733 | $159,279 | 25 | <$5M | AI Deep Dive |
| New Horizon Cuisine | Manufacturing | $156,068 | $157,701 | 17 | $6.0M | AI Deep Dive |
| Pietig Family Eyecare Pllc | Health Care and Social Assistance | $154,414 | $155,814 | 18 | <$5M | AI Deep Dive |
| Innovative Communication Technologies LLC | Construction | $153,312 | $153,984 | 20 | $6.8M | AI Deep Dive |
| Moravec Salons, LLC | Other Services (except Public Administration) | $153,168 | $154,486 | 24 | <$5M | AI Deep Dive |
| Trailhead Ventures, LLC | Accommodation and Food Services | $152,958 | $153,909 | 15 | <$5M | AI Deep Dive |
| Ankeny Urban Air LLC | Arts, Entertainment, and Recreation | $152,500 | $153,445 | 100 | $14.0M | AI Deep Dive |
| New Life Counseling PC | Health Care and Social Assistance | $152,495 | $155,846 | 16 | <$5M | AI Deep Dive |
| Moravec Salons LLC | Other Services (except Public Administration) | $150,652 | $151,890 | 18 | <$5M | AI Deep Dive |
| A & a Delivery LLC | Transportation and Warehousing | $150,000 | $151,011 | 23 | $6.3M | AI Deep Dive |
| A & a Delivery LLC | Transportation and Warehousing | $150,000 | $151,327 | 20 | <$5M | AI Deep Dive |
| Ankeny Auto Body, Inc. | Other Services (except Public Administration) | $149,900 | $150,437 | 12 | <$5M | AI Deep Dive |
| Thunderhead of Ankeny Inc. | Accommodation and Food Services | $148,874 | $150,863 | 25 | <$5M | AI Deep Dive |
| Keystone Church of Ankeny, Inc. | Other Services (except Public Administration) | $147,866 | $149,134 | 21 | N/A | AI Deep Dive |
| Wags Inc. | Other Services (except Public Administration) | $146,271 | $146,880 | 28 | $5.3M | AI Deep Dive |
| Wonderdogs, Inc. | Accommodation and Food Services | $146,264 | $147,246 | 48 | $8.5M | AI Deep Dive |