| Iowa Pacific Processors Inc. | Manufacturing | $558,550 | $562,430 | 54 | $19.1M | AI Deep Dive |
| Lint Van Lines Inc. | Transportation and Warehousing | $555,063 | $557,693 | 64 | $16.0M | AI Deep Dive |
| Sheet Metal Engineering, Inc. | Construction | $552,523 | $558,548 | 8 | $9.1M | AI Deep Dive |
| Sheet Metal Engineering Inc. | Construction | $552,522 | $556,170 | 28 | $8.9M | AI Deep Dive |
| Iowa Healthcare Collaborative | Finance and Insurance | $549,674 | $553,904 | 43 | N/A | AI Deep Dive |
| Business Publications Corp. INC | Information | $545,174 | $547,892 | 40 | $6.7M | AI Deep Dive |
| Miller Mechanical Specialties Inc. | Wholesale Trade | $544,980 | $549,264 | 35 | $10.7M | AI Deep Dive |
| Jethro and Jakes INC | Accommodation and Food Services | $544,614 | $550,955 | 62 | $11.0M | AI Deep Dive |
| Rj Conley Hotel Group Lc | Accommodation and Food Services | $540,190 | $545,622 | 83 | $26.0M | AI Deep Dive |
| Tom's Auto Sales & Service, Inc. | Retail Trade | $539,750 | $543,303 | 33 | $8.3M | AI Deep Dive |
| The Des Moines Embassy Club | Accommodation and Food Services | $538,164 | $541,629 | 43 | N/A | AI Deep Dive |
| Absolute Flavors LLC | Accommodation and Food Services | $538,000 | $542,746 | 103 | $18.3M | AI Deep Dive |
| Wakonda Club | Arts, Entertainment, and Recreation | $537,430 | $539,226 | 95 | $13.3M | AI Deep Dive |
| Exile Brewing Company, LLC | Manufacturing | $535,700 | $538,709 | 79 | $27.9M | AI Deep Dive |
| Full Court Press, Inc. | Accommodation and Food Services | $534,290 | $538,345 | 101 | $12.4M | AI Deep Dive |
| Centurion Stone of Iowa, Inc. | Wholesale Trade | $533,182 | $538,395 | 42 | $12.9M | AI Deep Dive |
| Central Service & Supply INC | Wholesale Trade | $528,475 | $529,899 | 30 | $9.2M | AI Deep Dive |
| Business Publications Corporation, Inc. | Information | $527,808 | $531,871 | 35 | $5.9M | AI Deep Dive |
| The Science Center of Iowa | Arts, Entertainment, and Recreation | $527,120 | $531,908 | 70 | N/A | AI Deep Dive |
| Science Center of Iowa | Professional, Scientific, and Technical Services | $527,100 | $532,752 | 70 | N/A | AI Deep Dive |
| Nelson Construction Services Co. | Construction | $526,920 | $529,028 | 28 | $31.9M | AI Deep Dive |
| Midwest Healthcare Linen Service LLC | Other Services (except Public Administration) | $526,500 | $530,928 | 80 | $15.3M | AI Deep Dive |
| Cruise Marketing, Inc. | Wholesale Trade | $525,000 | $530,119 | 54 | $13.9M | AI Deep Dive |
| Workgroups Davinci Inc. | Information | $522,992 | $526,333 | 15 | <$5M | AI Deep Dive |
| Obstetrical & Gynecological Associates of Des Moines Plc | Health Care and Social Assistance | $522,917 | $527,473 | 47 | $14.8M | AI Deep Dive |