| The Dana Company | Finance and Insurance | $384,800 | $387,344 | 27 | $13.9M | AI Deep Dive |
| Hale Supply Co | Wholesale Trade | $384,384 | $387,375 | 18 | <$5M | AI Deep Dive |
| Crinc, L.c. | Administrative and Support and Waste Management | $378,620 | $381,898 | 43 | $8.1M | AI Deep Dive |
| Grand View Christian School Assoc. Inc. | Educational Services | $378,058 | $381,334 | 68 | N/A | AI Deep Dive |
| Frank Rieman Music Co, Inc. | Retail Trade | $377,190 | $382,181 | 34 | $8.0M | AI Deep Dive |
| The Des Moines Embassy Club | Accommodation and Food Services | $376,700 | $380,797 | 43 | N/A | AI Deep Dive |
| Drake Diner LLC | Accommodation and Food Services | $376,344 | $378,426 | 19 | <$5M | AI Deep Dive |
| Cleaning Connection, Inc. | Administrative and Support and Waste Management | $375,800 | $378,940 | 109 | $23.6M | AI Deep Dive |
| Nursepro Staffing Agency | Health Care and Social Assistance | $375,000 | $376,875 | 85 | $14.6M | AI Deep Dive |
| Badawi Pizza Company INC | Accommodation and Food Services | $375,000 | $377,301 | 85 | $15.1M | AI Deep Dive |
| Food Bank of Iowa | Health Care and Social Assistance | $375,000 | $377,563 | 39 | N/A | AI Deep Dive |
| Saddlery Restaurants, INC | Accommodation and Food Services | $374,917 | $377,793 | 29 | $5.1M | AI Deep Dive |
| Jethros Pork Chop Grill INC | Accommodation and Food Services | $373,635 | $377,771 | 45 | $8.0M | AI Deep Dive |
| Jdb Auto Sales, Inc. | Retail Trade | $371,700 | $375,706 | 28 | $7.1M | AI Deep Dive |
| Sem Insurance Brokers INC | Finance and Insurance | $369,717 | $371,784 | 41 | $21.1M | AI Deep Dive |
| Des Moines Symphony Association | Arts, Entertainment, and Recreation | $369,471 | $373,368 | 21 | N/A | AI Deep Dive |
| Real Optics INC | Retail Trade | $368,872 | $372,975 | 48 | $8.4M | AI Deep Dive |
| Todd's Bbi International, INC | Manufacturing | $368,300 | $373,200 | 48 | $16.9M | AI Deep Dive |
| Midwest Ambulance Service of Iowa Inc. | Health Care and Social Assistance | $367,968 | $370,268 | 41 | $9.5M | AI Deep Dive |
| Beer, Inc. | Accommodation and Food Services | $366,832 | $368,938 | 87 | $10.7M | AI Deep Dive |
| Rocket Referrals | Professional, Scientific, and Technical Services | $365,401 | $368,214 | 24 | <$5M | AI Deep Dive |
| For Sure Roofing LLC | Construction | $364,800 | $366,908 | 50 | $16.5M | AI Deep Dive |
| Chl Development Co. | Accommodation and Food Services | $364,722 | $367,285 | 36 | $6.4M | AI Deep Dive |
| Midwest Ambulance Service of I | Health Care and Social Assistance | $364,300 | $367,619 | 25 | $5.8M | AI Deep Dive |
| Des Moines Symphony Association | Arts, Entertainment, and Recreation | $364,100 | $367,113 | 20 | N/A | AI Deep Dive |