| Carroll Distributing and Construction Supply Esop | Wholesale Trade | $3,897,600 | $3,942,422 | 316 | $96.8M |  Research |
| Winger Contracting Company | Other Services (except Public Administration) | $3,500,000 | $3,542,863 | 336 | $72.2M | Research |
| River Hills Community Health Center, Inc. | Health Care and Social Assistance | $1,755,865 | $1,770,874 | 157 | N/A | Research |
| Norris Asphalt Paving Co., Lc | Construction | $1,530,800 | $1,542,417 | 113 | $35.0M | Research |
| Winbco Tank Company | Manufacturing | $1,364,200 | $1,381,745 | 89 | $21.8M | Research |
| Winbco Tank Company | Manufacturing | $1,364,200 | $1,386,785 | 194 | $50.8M | Research |
| Elliot Oil Company | Retail Trade | $1,046,382 | $1,051,033 | 220 | $73.6M | Research |
| Douds Stone, LLC | Mining, Quarrying, and Oil and Gas Extraction | $1,029,900 | $1,037,716 | 71 | <$5M | Research |
| Elliot Oil Company | Mining, Quarrying, and Oil and Gas Extraction | $991,995 | $997,699 | 220 | $13.3M | Research |
| Tenco Industries Inc. | Health Care and Social Assistance | $872,500 | $857,204 | 152 | N/A | Research |
| Vaughn Chevrolet | Retail Trade | $762,800 | $767,843 | 69 | $17.4M | Research |
| Hite Trucking, LLC | Transportation and Warehousing | $676,032 | $680,163 | 68 | $13.7M | Research |
| Southern Iowa Mental Center | Health Care and Social Assistance | $651,170 | $656,506 | 70 | $12.5M | Research |
| C & C Manufacturing, LLC | Manufacturing | $592,744 | $597,843 | 45 | $12.6M | Research |
| Pizza Brake, INC | Accommodation and Food Services | $464,696 | $468,272 | 155 | $27.5M | Research |
| Ottumwa Development, Inc. | Health Care and Social Assistance | $434,087 | $436,688 | 57 | $9.7M | Research |
| Ottumwa Development INC | Health Care and Social Assistance | $434,087 | $437,548 | 77 | $13.1M | Research |
| Southern Iowa Mechanical LLC | Construction | $415,123 | $420,508 | 41 | $14.0M | Research |
| Clemons INC of Ottumwa | Retail Trade | $386,612 | $391,262 | 45 | $11.8M | Research |
| Christner Contracting, INC | Construction | $380,605 | $382,899 | 24 | $27.3M | Research |
| Jdr Farms Trucking LLC | Transportation and Warehousing | $362,500 | $365,420 | 31 | $8.3M | Research |
| American Home Finding Association | Health Care and Social Assistance | $350,000 | $352,013 | 70 | N/A | Research |
| Vaughn Chrysler-Jeep-Dodge | Retail Trade | $349,600 | $351,989 | 32 | $8.1M | Research |
| Bowling Motors | Retail Trade | $336,600 | $341,219 | 25 | $6.5M | Research |
| Parkview Plaza | Accommodation and Food Services | $322,220 | $323,786 | 45 | $14.1M | Research |