| Wapello Rural Water Association, Inc. | Utilities | $310,193 | $313,329 | 18 | N/A |  Research |
| Geroge Boitnott Enterprises, Inc. | Administrative and Support and Waste Management | $292,000 | $294,831 | 24 | <$5M | Research |
| Supreme Staffing | Administrative and Support and Waste Management | $289,277 | $293,406 | 59 | $9.3M | Research |
| 100x Marketing Group, Inc. | Professional, Scientific, and Technical Services | $284,171 | $286,002 | 19 | <$5M | Research |
| 100x Marketing Group INC | Arts, Entertainment, and Recreation | $284,170 | $285,520 | 19 | <$5M | Research |
| Atlantis Penn Place Opco LLC | Health Care and Social Assistance | $251,165 | $253,284 | 38 | $6.5M | Research |
| Young Men's Christian Association of Ottumwa Iowa | Arts, Entertainment, and Recreation | $240,480 | $243,266 | 97 | N/A | Research |
| Young Mens Christioan Association of Ottumwa Iowa | Arts, Entertainment, and Recreation | $236,335 | $237,228 | 158 | N/A | Research |
| Parkview Plaza | Accommodation and Food Services | $230,157 | $231,448 | 45 | $14.1M | Research |
| Nobrake Pizza, Inc. | Accommodation and Food Services | $221,472 | $222,752 | 55 | $9.8M | Research |
| Hospice | Health Care and Social Assistance | $218,617 | $219,668 | 28 | <$5M | Research |
| Anderson Larkin & Co PC | Professional, Scientific, and Technical Services | $208,320 | $209,275 | 17 | $5.4M | Research |
| Anderson Larkin & Co., Ppc | Professional, Scientific, and Technical Services | $195,900 | $197,581 | 16 | $5.1M | Research |
| South Side Drug Inc. | Retail Trade | $191,700 | $193,180 | 22 | <$5M | Research |
| Poole's Truck Service, INC | Other Services (except Public Administration) | $179,800 | $180,795 | 25 | <$5M | Research |
| Yates and Yates Glass Company, INC | Construction | $169,900 | $171,099 | 16 | <$5M | Research |
| Greater Ottumwa Partners in Progress | Public Administration | $168,105 | $169,539 | 11 | N/A | Research |
| Bridge City Sanitation, LLC | Administrative and Support and Waste Management | $165,200 | $166,480 | 26 | <$5M | Research |
| Midwest Solid Surfaces, LLC | Manufacturing | $161,000 | $162,060 | 15 | $14.9M | Research |
| Harrison, Moreland, Webber, Simplot & Maxwell | Professional, Scientific, and Technical Services | $159,460 | $160,375 | 11 | <$5M | Research |
| Crown Distributin Co | Manufacturing | $153,992 | $154,989 | 11 | <$5M | Research |
| Ottumwa Pizza Ranch LLC | Accommodation and Food Services | $150,400 | $151,507 | 20 | <$5M | Research |
| O-Town Communications, Inc. | Information | $148,600 | $150,261 | 20 | <$5M | Research |
| Harrison Moreland Webber & Simplot P.c. | Professional, Scientific, and Technical Services | $146,383 | $147,570 | 11 | <$5M | Research |
| Dwayne J. Patritto DDS Ms Plc | Health Care and Social Assistance | $146,167 | $146,975 | 10 | <$5M | Research |