| B & W Custom Truck Beds INC | Manufacturing | $4,495,400 | $4,547,721 | 391 | $95.6M |  Research |
| Southeast Kansas Mental Health Center | Health Care and Social Assistance | $1,329,100 | $1,337,208 | 151 | N/A | Research |
| Whitaker Companies INC | Mining, Quarrying, and Oil and Gas Extraction | $672,800 | $678,855 | 51 | <$5M | Research |
| Hofer & Hofer & Associates Inc. | Construction | $116,189 | $116,496 | 11 | <$5M | Research |
| Hofer & Hofer & Associates, INC | Construction | $112,800 | $113,890 | 12 | $13.7M | Research |
| Humboldt Industries, Inc. | Manufacturing | $109,500 | $110,181 | 10 | <$5M | Research |
| Broyles Petroleum Equipment Co | Construction | $100,790 | $101,540 | 7 | <$5M | Research |
| Kevin W Heisler | Transportation and Warehousing | $85,100 | $85,674 | 13 | <$5M | Research |
| Humboldt Daycare and Preschool INC DBA the Growing Place Chi | Health Care and Social Assistance | $78,800 | $79,251 | 19 | <$5M | Research |
| Kevin Heisler | Transportation and Warehousing | $72,490 | $72,756 | 13 | <$5M | Research |
| M. Sean Mcreynolds, Dds, LLC | Health Care and Social Assistance | $71,180 | $71,465 | 7 | <$5M | Research |
| M. Sean Mcreynolds DDS LLC | Health Care and Social Assistance | $71,100 | $71,531 | 7 | <$5M | Research |
| Humboldt Assisted Living, LLC | Health Care and Social Assistance | $63,000 | $63,533 | 17 | <$5M | Research |
| Opie's Family Resturant INC | Accommodation and Food Services | $49,226 | $49,665 | 24 | <$5M | Research |
| Weilert Chiropractic Clinic, LLC | Health Care and Social Assistance | $43,400 | $43,652 | 3 | <$5M | Research |
| Opie's Family Restaurant, INC | Accommodation and Food Services | $35,100 | $35,380 | 24 | <$5M | Research |
| James L. Jarred | Agriculture, Forestry, Fishing and Hunting | $30,800 | $30,972 | 3 | <$5M | Research |
| D&d Propane INC | Retail Trade | $30,000 | $30,120 | 6 | <$5M | Research |
| Irishman Excavating | Transportation and Warehousing | $29,113 | $29,270 | 13 | <$5M | Research |
| Dean A. Rinehart | Construction | $28,700 | $29,016 | 5 | <$5M | Research |
| D & D Propane, Inc. | Retail Trade | $28,398 | $28,566 | 6 | <$5M | Research |
| Humboldt Transfer LLC | Transportation and Warehousing | $23,448 | $23,603 | 3 | <$5M | Research |
| Eastside Tire Inc. | Retail Trade | $22,250 | $22,389 | 4 | <$5M | Research |
| Eastside Tire INC | Retail Trade | $22,110 | $22,224 | 4 | <$5M | Research |
| Saint Peter Lutheran Church | Other Services (except Public Administration) | $22,000 | $22,118 | 6 | N/A | Research |