| Uncle Bubba's Barbecue and Smokehouse | Accommodation and Food Services | $162,743 | $164,901 | 41 | $7.3M |  Research |
| Cleveland Construction | Construction | $161,087 | $161,995 | 13 | <$5M | Research |
| Craigen's Garage, LLC | Other Services (except Public Administration) | $160,100 | $161,780 | 19 | <$5M | Research |
| Craigen's Garage LLC | Other Services (except Public Administration) | $160,080 | $161,361 | 21 | <$5M | Research |
| Hernando United Methodist Church | Other Services (except Public Administration) | $159,753 | $160,835 | 57 | N/A | Research |
| Forest to Floor | Retail Trade | $157,600 | $148,049 | 16 | <$5M | Research |
| Funderburk's Pharmacy | Retail Trade | $153,800 | $154,748 | 15 | <$5M | Research |
| Freight Management Experts | Retail Trade | $152,100 | $153,646 | 46 | $7.2M | Research |
| Cooke Insurance Center Inc. | Finance and Insurance | $151,400 | $136,513 | 20 | $10.3M | Research |
| Spms&n-Desoto, LLP | Professional, Scientific, and Technical Services | $148,377 | $124,435 | 6 | <$5M | Research |
| Lamb Construction & Equipment Service LLC | Construction | $142,190 | $142,676 | 16 | <$5M | Research |
| National Tank, Inc. | Manufacturing | $140,986 | $141,840 | 10 | <$5M | Research |
| Hernando Hotel Group LLC | Accommodation and Food Services | $140,679 | $142,394 | 24 | $7.5M | Research |
| William Crumley | Accommodation and Food Services | $139,894 | $140,963 | 61 | $10.8M | Research |
| Ac Management Group LLC | Accommodation and Food Services | $137,874 | $138,295 | 34 | $6.0M | Research |
| Affordable Medical Care LLC | Health Care and Social Assistance | $136,112 | $137,689 | 19 | <$5M | Research |
| Eldridge Services INC | Construction | $134,865 | $136,094 | 12 | <$5M | Research |
| Cf Wright, Inc. | Accommodation and Food Services | $130,700 | $131,648 | 23 | <$5M | Research |
| Desoto County Animal Clinic PA | Professional, Scientific, and Technical Services | $129,100 | $129,893 | 18 | <$5M | Research |
| Hernando Dental Group | Health Care and Social Assistance | $127,302 | $128,571 | 10 | <$5M | Research |
| J. Cox Orthodontics, Pllc | Health Care and Social Assistance | $124,330 | $125,456 | 13 | <$5M | Research |
| Reliable Equipment LLC | Other Services (except Public Administration) | $124,050 | $125,311 | 13 | <$5M | Research |
| Advanced Collision INC | Other Services (except Public Administration) | $124,000 | $125,150 | 13 | <$5M | Research |
| Ac Management Group II LLC | Accommodation and Food Services | $123,811 | $124,285 | 24 | <$5M | Research |
| Spms&n- Desoto LLP | Professional, Scientific, and Technical Services | $122,633 | $123,341 | 6 | <$5M | Research |